BioTechniques

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  Año 2000, Vol. 29, Número 1

  • Detection of Protein-Protein Interactions Using a Green Fluorescent Protein-Based Mammalian Two-Hybrid System

    S. Rupp, F.-J. Johannes, M. Fotin-Mleczek, M. Rottmann, G. Rehg

    págs. 22-25

    

  • Titration of Infective and Noninfective Ff Filamentous Bacteriophages Using a Monoclonal Antibody against g3p

    M. Tesar, C. Frisch, A. Knappik, M. Choidas

    págs. 26-29

    

  • Contact Printing of Lift Filters for Alignment of Plaque and Colony Lifts

    M. W. Frohlich

    págs. 30-33

    

  • Wrapper Arms Strategy to Construct Multicomponent Plasmids Using Three-Piece Ligation

    K. Hagiwara, M. G. McMenamin

    págs. 34-37

    

  • DNase I Activity Retained after Heat Inactivation in Standard Buffer

    T. Odawara, H. Yoshikura, K. Hanaki, H. Nakatake, K. Yamamoto

    págs. 38-41

    

  • Trypan Blue as a Slow Migrating Dye for SSCP Detection in Polyacrylamide Gel Electrophoresis

    H. Igarashi, K. Nagura, H. Sugimura

    págs. 42-43

    

  • CEL I Enzymatic Mutation Detection Assay

    A. K. Godwin, A. T. Yeung, J. Kulinski, D. Besack, C. A. Oleykowski

    págs. 44-47

    

  • Modified Primers Facilitate Rapid Screening of 16S rRNA Gene Libraries

    J. R. Marchesi, A. J. Weightman

    págs. 48-51

    

  • Preparation of PCR-Quality Mouse Genomic DNA with Hot Sodium Hydroxide and Tris (HotSHOT)

    A. A. Truett, J. A. Walker, M. L. Warman, G. E. Truett, P. Heeger, Randall L. Mynatt

    págs. 52-53

    

  • Choice of Microcentrifuge Tubes Influences T Cell Proliferation Assay

    M. Fried, A. D. Christ, Y. Wei, R. J. Mrsny, R. S. Blumberg

    págs. 54-59

    

  • Multiple Sequence Alignment Tools on the Web

    G. J. Gaskell

    págs. 60-69

    

  • Scientific Imaging with Digital Cameras

    K. R. Spring

    págs. 70-77

    

  • Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II

    L. R. Bernardi, G. De Bellis, C. Battaglia, G. Salani, C. Consolandi

    págs. 78-80

    

  • Transmembrane Motility Assay of Transiently Transfected Cells by Fluorescent Cell Counting and Luciferase Measurement

    D. Theodorescu, J. J. Gildea, M. A. Harding, K. M. Gulding

    págs. 81-87

    

  • Quantification of Enterovirus RNA in Sludge Samples Using Single Tube Real-Time RT-PCR

    L. Schwartzbrod, V. Marechal, J.-C. Nicolas, S. Billaudel, V. Ferre, S. Monpoeho, A. Dehee, B. Mignotte

    págs. 88-93

    

  • Sequence Alterations Can Mask Each Other's Presence during Screening with SSCP or Heteroduplex Analysis: BRCA Genes as Examples

    T. I. Orban, B. Csokay, E. Olah

    págs. 94-99

    

  • Color and Graphic Display (CGD): Programs for Multiple Sequence Alignment Analysis in Spreadsheet Software

    C. Delamarche

    págs. 100-107

    

  • Fingerprinting Method for Phylogenetic Classification and Identification of Microorganisms Based on Variation in 16S rRNA Gene Sequences

    P. Agrawal, G. P. S. Raghava, R. J. Solanki, V. Soni

    págs. 108-117

    

  • Software for Quantitation and Visualization of Expression Array Data

    D. Strehlow

    págs. 118-121

    

  • Virtual Nitrogen Tank to Monitor Frozen Cell Stocks

    A. Zaldumbide, K. E. Boulukos, P. Pognonec

    págs. 122-127

    

  • Antibody Engineering at the Millennium

    J. V. Gavilondo, J. W. Larrick

    págs. 128-145

    

  • The Admid System: Generation of Recombinant Adenoviruses by Tn7-Mediated Transposition in E. coli

    M. P. Weiner, C. A. Richards, C. E. Brown, J. P. Cogswell

    págs. 146-155

    

  • Rapid Colchicine Competition-Binding Scintillation Proximity Assay Using Biotin-Labeled Tubulin

    S.-C. Ng, S. K. Tahir, P. Kovar, S. H. Rosenberg

    págs. 156-161

    

  • Method for Cloning In Vivo Targets of the Egr-1 Transcription Factor

    I. de Belle, D. Mercola, E. D. Adamson

    págs. 162-169

    

  • Cell-Based, High-Content Screen for Receptor Internalization, Recycling and Intracellular Trafficking

    R. L. DeBiasio, B. R. Conway, L. K. Minor, K. T. Demarest, R. N. Ghosh, Y.-T. Chen, R. DeBiasio

    págs. 170-175