BioTechniques

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  Año 1999, Vol. 27, Número 5

  • MboII endonuclease heat inactivation before agarose gel electrophoresis to prevent artifactual bands in restriction patterns

    G.M. Giammanco, F. Grimont, P.A.D. Grimont

    págs. 886-887

    

  • Electrophoretic mobility shift assay coupled with immunoblotting for the identification of DNA-binding proteins

    B.K Hurlburt, T.C. Chambers, M.T. Osborn, K. Herrin, F.G. Buzen

    págs. 887-892

    

  • Plasmid topologies that enhance the transformation efficiency of yeast

    C. K. Raymond, V.R. Mugford, S. L. Sexson

    págs. 892-896

    

  • Improved β-glucuronidase reporter for mammalian gene expression analysis

    S. Chan, J. Plumb, J. Hu

    págs. 896-900

    

  • Chloramphenicol acetyltransferase expression as a sensor for fusion activity

    D. Gicklhorn, M. Eickmann, K. Radsak

    págs. 902-908

    

  • Infecting larval arthropods with a chimeric, double subgenomic sindbis virus vector to express genes of interest

    K.E. Olson, B.J Beaty, S. Higgs, C.T. Oray, K. Myles

    págs. 908-911

    

  • Transient conversion to RecA+ phenotype to permit P1 transduction in any escherichia coli recA-Strains

    M. Sektas, M. Gregorowicz, W. Szybalski

    págs. 911-914

    

  • Limited T4 exonuclease activity and partial fill-in expand insertion site options for PCR subcloning

    S.B. Sharp, N. Green, S. Vu, S. Farahmand

    págs. 914-16

    

  • Quality control PCR: a method for detecting inhibitors of Taq DNA polymerase

    R.A. Reiss, B. Rutz

    págs. 920-926

    

  • Silver-stained comet assay for detection of apoptosis

    C. Ferrarotto, J.R.N. McLean, J.P. McNamee, N. Kizilian, R.C. Wilkins, P. Reinhardt

    págs. 926-930

    

  • Rapid screening of elution conditions prior to immunopurification of proteins

    S.M. Rutkowska, P.M. Skowron

    págs. 930-934

    

  • In Vivo measurement of biodistribution kinetics of radiolabeled compounds in laboratory animals

    C. Gottstein, R. Forde

    págs. 934-938

    

  • Internet on-ramp: Intellectual and pattent resources on the internet

    D. M. Sander

    págs. 940-942

    

  • Construct for high-level expression and low misincorporation of Lysine for Argininge during expression of pET-encoded eukaryotic proteins in Escherichia coli

    J. You, R.E. Cohen, C.M. Pickart

    págs. 950-954

    

  • Modulating restriction endonuclease activities and specificities using neutral detergents

    C.M. Dupureur, L.H Conlan, T.J. José, K.C. Thornton

    págs. 955-960

    

  • Reverse transcription in the presence of dideoxynucleotides to increase the sensitivity of expression monitoring with cDNA arrays

    G. Piétu, C. Decaene, I. Reguigne-Arnould, C. Auffray

    págs. 962-966

    

  • RT-PCR: characterization of long multi-gene operons and multiple transcript gene clusters in bacteria

    A. Gupta

    págs. 966-972

    

  • High-density small-volume gel loading directly from capillary tubes

    E.E. Dixon, H.T. Evensen, D. R. Meldrum, C. Saenphimmachak

    págs. 974-978

    

  • Isolation of pure human mucosal epithelium for RNA analysis

    T. Ohnishi, K. Okubo

    págs. 978-986

    

  • Biofeedback

    págs. 988-988

    

  • Confocal laser scanning microscopy

    S.W. Paddock

    págs. 992-1004

    

  • Single-molecule fluorescence observed with mercury lamp illumination

    H.A. Lester, S.R. Quake, M. Unger, E. Kartalov, C.-S. Chiu

    págs. 1008-1014

    

  • Denaturing gradient gel electrophoresis (DGGE): a rapid and sensitive technique to screen nucleotide sequence variation in populations

    R.E. Withler, K.M. Miller, T.J. Ming, A.D. Schulze

    págs. 1016-1030

    

  • Optimized RNA gel-shift and UV cross-linking assays for characterization of cytoplasmic RNA-protein interactions

    J.M. Staton, P.J. Leedman, A.M. Thomson, J.T. Rogers, C.E. Walker

    págs. 1032-1042

    

  • Multiplex relative RT-PCR method for verification of differential gene expression

    W.E. Spencer, M.J. Christensen

    págs. 1044-1052

    

  • Genotyping the babon ABO histo-blood group locus by two-color fluorescence SSCP

    A.A. Szalay, D.C. Diamond, K. Illes, L.L. Bailey

    págs. 1054-1062

    

  • Cisplatin-digoxigenim mRNA labeling for nonradioactive detection of mRNA hybridized onto nucleic acid cDNA arrays

    T. Hoevel, H. Holz, M. Kubbies

    págs. 1064-1067

    

  • New products

    págs. 1068-1079

    

  • Index to advertisers

    págs. 1078-1078