BioTechniques

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  Año 1999, Vol. 26, Número 1

  • Generation of Single-Stranded DNA Hybridization Probes by PCR Using a Short, Synthetic DNA Template

    T. P. Condon

    pág. 18

    

  • Method to Distinguish Between the MMAC1/PTEN Gene and Its Pseudogene in RT-PCR Analysis of Point Mutations

    J. Liu, J. Kagan

    pág. 19

    

  • Use of a Promoterless Renilla Luciferase Vector as an Internal Control Plasmid for Transient Co-Transfeetion Assays of Ras-Mediated Transcription Activation

    G. Behre, L. T. Smith, D. G. Tenen

    pág. 24

    

  • High-Level Expression of Human Histone H4 in E. Coli

    V. L. Burnett, D. L. Springer

    pág. 30

    

  • When Less is More: Enhanced Baculovirus Production of Recombinant Proteins at Very Low Multiplicities of Infection

    P. M. Steed, J. M. Liebman, D. LaSala, W. Wang

    pág. 36

    

  • Normalization of RNA Hybridization Signals by Means of SYBR[R] Green II-Stained 28S or 18S Ribosomal RNA and a Phosphor Imager

    A.-N. Spiess, R. Ivell

    pág. 46

    

  • Procedure for the Investigation of Bacterial Genomes: Random Shot-Gun Cloning, Sample Sequencing and Mutagenesis of Campylobacter jejuni

    B. W. Wren, A. V. Karlyshev, J. Henderson, J. M. Ketley

    pág. 50

    

  • Quantitation of á-Galactosidase from Yeast Cells Using a Chemiluminescent Substrate

    M. Nevels, H. Wolf, T. Dobner

    pág. 57

    

  • Isolation of High-Molecular-Weight Genomie DNA from Intact Biohazardous Mammalian Tissues

    V. Liska, R. M. Ruprecht

    pág. 62

    

  • Rapid Minipreparation of Plasmid DNA for Screening Multiple Colonies

    A. R. Davis, P. L. Mascolo, M. T. Quinn

    pág. 66

    

  • World Wide Web Resources for Microbiologists

    L. Blaine, P. Karp, K. Sanderson, D. P. Nierlich, D. Benson, M. K. B. Berlyn

    pág. 70

    

  • Detection of Alexandrium tamarensis by Rapid PCR Analysis

    S. T. Haley, J. F. Cavender, T. E. Murray

    pág. 88

    

  • Enhancement of PCRs by Partial Restriction Digestion of Genomic Templates

    Raymond Vazquez, Mark L. Steinberg

    pág. 91

    

  • Synthesis and Automated Detection of Fluorescently Labeled Primer Extension Products

    E. Altermann, J. R. Klein, B. Henrich

    pág. 96

    

  • Flow Cytometric Analysis of the Cell Cycle in Transfected Cells Without Cell Fixation

    D. G. Pestov, M. Polonskaia, L. E. Lau

    pág. 102

    

  • Recombinant Polyketide Synthesis in Streptomyces: Engineering of Improved Host,Strains

    R. Ziermann, M. C. Betlach

    pág. 106

    

  • Quantitative RT-PCR: Pitfalls and Potential

    W. M. Freeman, S. J. Walker, K. E. Vrana

    pág. 112

    

  • Long RT-PCR Amplification of the Entire Coding Sequence of the Polycystic Kidney Disease 1 (PKD1) Gene

    P.-T. Yenchitsomanus, W. Thongnoppakhun, P. Wilairat, K. Vareesangthip

    pág. 126

    

  • General Method for Plasmid Construction Using Homologous Recombination

    C. K. Raymond, T. A. Pownder, S. L. Sexson

    pág. 134

    

  • Fusion Proteins Could Generate False Positives in Peptide Phage Display

    D. Banville, K. K. Murthy, I. Ekiel, S.-H. Shen

    pág. 142

    

  • Nonradioactive Detection of Differentially Expressed Genes Using Complex RNA or DNA Hybridization Probes

    T. Laengin, A. B. Reske-Kunz, R. Ross, X.-L. Ross, B. Rueger

    pág. 150