BioTechniques

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  Año 2002, Vol. 32, Número 6

  • BioSpotlight

    pág. 1209

    

  • RNase H and its effects on PCR

    S.K. Polumuri, A. Ruknudin, D.H. Schulze

    págs. 1224-1225

    

  • Automated high-throughput RNA analysis by capillary electrophoresis

    A. Guttman, J. Khandurina, H.-S. Chang, B. Wanders

    págs. 1226-1230

    

  • Construction of expression vectors to produce affinity-tagged proteins in Pseudomonas

    R. Couch, H. Seidle, R. Parry

    págs. 1230-1236

    

  • Synthesis of proteins with disulfide bonds in E. coli using defined culture media

    Javier Varela, G. Giménez-Gallego, Carlos Fernández Tornero, A. Ramón, M.L. Navarro

    págs. 1238-1242

    

  • Improved blunt-end cloning by replacing EcoRV with Eco321

    R. Koesters, M. von Knebel Doeberitz

    págs. 1244-1246

    

  • Inhibition of bacterial pyrodoxal-depending enzymes by (aminooxy)-acetic acid improves selective 15N isotope labeling of bacterially expressed protein

    L.V. Lopukhov, A.A. Ponomareva, L.O. Yagodina

    págs. 1248-1250

    

  • Amyloglucosidase suppresses interference by glycogen in the quantification of DNA using the Hoechst 33258 dye

    D. Renz, P. Maurer, W. Doerfler, L. Palka, M.E. Palka-Santini, S. Chambon

    págs. 1250-1256

    

  • Microscale assay monitors algal growth characteristics

    K. K. Bernd, N. Cook

    págs. 1256-1259

    

  • Dynamic imaging using fluorescence resonance energy transfer

    M. Elangovan, R. N. Day, A. Periasamy

    págs. 1260-1265

    

  • One-step, highly efficient site-directed mutagenesis by toxic protein selection

    C.R. Ruprecht, F. Wong-Staal, B.A. McFadden, T.R. Reddy, R. M. Ruprecht, W. Xu, Y. Zhang, L.-Y. Yeh

    págs. 1266-1270

    

  • Minimal FLAG sequence useful in the functional epitope tagging of H-Ras

    K.Y. Johnson, L. Liu, T.S. Vincent

    págs. 1270-1280

    

  • High-level expression of recombinant Fc chimeric proteins in suspension cultures of stably transfected J558L cells

    C.J. McNamee, D.J. Moss, M.R. Howard, A.P. Lodge, J.E. Reed

    págs. 1282-1288

    

  • Analysis of the activity of nuclear export signals using fluorescent BSA conjugates

    C.M. Feldherr, D. Akin

    págs. 1288-1296

    

  • Magnetic bead purification of labeled DNA fragments for high-throughput capillary electrophoresis sequencing

    D. Humphries, M. Pollard, N. Hammon, T. Hawkins, C. Elkin, H. Kapur, T. Smith

    págs. 1296-1302

    

  • Toward a universal standard: comparing two methods for standardizing spotted microarray data

    M.R. Weil, T. Macatee, H.R. Garner

    págs. 1310-1314

    

  • Local mean normalization of microarray element signal intensities across an array surface: quality control and correction of spatially systematic artifacts

    J. Pevsner, C. Colantuoni, G. Henry, S. Zeger

    págs. 1316-1320

    

  • Creating arrays by centrifugation

    M. Jobs, W.M. Howell, A.J. Brookes

    págs. 1322-1329

    

  • RNA amplification results in reproducible microarray data with slight ratio bias

    P. Van Hummelen, L.G. Puskás, A. Zvara, L. Hackler jr.

    págs. 1330-1340

    

  • Conditions to ensure competitive hybridization in two-color microarray: a theoretical and experimental analysis

    S. Ghosh, Y. Wang, X. Wang, S.-W. Guo

    págs. 1342-1346

    

  • Monitoring of representational difference analysis subtraction procedures by global microarrays

    J. Odeberg, J. Quackenbush, J. Lundeberg, T. Andersson, P. Unneberg, P. Nilsson

    págs. 1348-1358

    

  • High-throughput array production using precision glass syringes

    N. Nishimura, Y. Maruta, Y. Hashimoto, D. Wright, H.-C. Wu, A. Azarani, J. Shieh, C. To, J. Carramao

    págs. 1360-1365

    

  • Loader lite: a new software tool for the ABI PRISM (R) 2700 DNA sequencer

    J.T. Warren, C.L. Kovár, S.E. Scherer, P.H. Havlak, R. A. Gibbs, G.B.I. Scott, D.L. Steffen, D. Edgar

    págs. 1366-1371

    

  • Processing of gene expression data generated by quantitative real-time RT-PCR

    Z. Dobbie, P.Y. Muller, H. Janovjak, A.R. Miserez

    págs. 1372-1379

    

  • Computing fuzzy associations for the analysis of biological literature

    M.A. Andrade, C. Perez-Iratxeta, H.S. Keer, P. Bork

    págs. 1380-1385

    

  • Automatic quantitation of hybridization signals on cDNA arrays

    O. Alibert, H. Guiot, C. Auffray, G. Piétu, F. Tahi, B. Achddou, C. Decraene

    págs. 1386-1397

    

  • Microarray Assistant clone organizer and array simulator

    R. Anbazhagan

    págs. 1398-1402

    

  • Use of degenerate primers and touchdown PCR for construction of cDNA libraries

    J.L.R. Fietto, R. DeMarco, S. Verjovski-Almeida

    págs. 1404-1411

    

  • Persistent DNA contamination in competitive RT-PCR using cRNA internal standards: identity, quantity, and control

    J.L.K. Matthews, M. Chung, J.R. Matyas

    págs. 1412-1417

    

  • 2002 author index (volume32)

    págs. 1418-1421