BioTechniques

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  Año 2002, Vol. 33, Número 4

  • BioSpotlight

    pág. 713

    

  • Ni+-affinity purification of untagged cAMP receptor protein

    J.R. Wickstrum, S.M. Egan

    págs. 728-730

    

  • Assay for intracellular calcium using a codon-optimized aequorin

    W.I. Vernon, J.A. Printen

    págs. 730-734

    

  • Accelerated apoptotic DNA laddering protocol

    M.C. Yeung

    págs. 734-736

    

  • High-efficiency cloning system for versatile adaptation of DNA fragments

    J. Dekker, M. Badowski, D.T. Harris, P. Luo, T.C. Tsang, C. Takeuchi

    págs. 738-742

    

  • Serial competitive RT-PCR using multiple standards

    M. Szibor, H. Morawietz

    págs. 744-748

    

  • Simultaneous detection of co-segregating missense mutations of the toll-like receptor 4

    D. Haering, C. Mannhalter, K. Hsieh, G. Endler, W. Lalouschek

    págs. 748-754

    

  • Single-step isolation of N-linked glycans from deglycosylation reaction mixtures

    M.A. Snodgrass, A.J. Jesaitis, R.M. Taylor, T.R. Foubert, J.B. Burritt

    págs. 754-758

    

  • Benchtop and microcentrifuge preparation of Pseudomonas aeruginosa competent cells

    R. Chuanchuen, C.T. Narasaki, H. P. Schweizer

    págs. 760-763

    

  • Agar plug/serial dilution approach for rapid PCR screening of phage libraries

    D. Vaiman

    págs. 764-766

    

  • Pseudogene-free amplification of human GAPDH cDNA

    H.H. Sigusch, M.H. Lehmann, J. Weber, O. Gastmann

    págs. 766-770

    

  • Basic principles of microscope objectives

    M.W. Davidson, M. Abramovitz, K. R. Spring, H.E. Keller

    págs. 772-781

    

  • In vivo cloning strategy for Rhizobium plasmids

    P. Chain, E. Martínez-Romero, Ismael Hernández Lucas, Patrick Mavingui, T. Finan

    págs. 782-788

    

  • Yeast tRNA as carrier in the isolation of microscale RNA for global amplification and expression profiling

    R. W. Davis, Q. Tian Wang, Xiao Wenzhong, M. Mindrinos

    págs. 788-796

    

  • Microplate diffusion assay for screening of ß-glucanase-producing microorganisms

    J.L. Zantinge, H.C. Huang, K.J. Cheng

    págs. 798-806

    

  • Bacteriophage gene targeting vectors generated by transplacement

    K. Ishidate, D.E. Rancourt, C. Aoyama, K. Woltjen, F.C. Mansergh

    págs. 806-812

    

  • SNPCEQer: detecting SNPs in sequences generated by the Beckman CEQ (TM) 2000 DNA Analysis System

    A. Pertsemlidis, H.R. Garner, E.M. Flood, F. Tang, M.M. Horvath

    págs. 814-822

    

  • SNPkit: an efficient approach to systematic evaluation of candidate single nucleotide polymorphisms in public databases

    J. Li, X. Xu, K. Hao, T. Niu, C. Sangokoya

    págs. 822-830

    

  • Directional genome walking using PCR

    S.K. Sopory, M.K. Reddy, R.N. Mishra, S.L. Singla-Pareek, S. Nais

    págs. 830-834

    

  • Real-time impedance assay to follow the invasive activities of metastatic cells in culture

    I. Giaever, C.R. Keese, K. Bhawe, J. Wegener

    págs. 842-850

    

  • In vivo functional proteomics: mammalian genome annotation using CD-tagging

    L. Hennen, C. Hauser, S. Adler, P.B. Berget, J.W. Jarvik, G.W. Fisher, C. Shi

    págs. 852-866

    

  • Mammalian expression cloning of nucleic acid binding proteins by agarose thin-layer gelshift clone selection

    A. Owusu, D.V. Agoston, A. Dobi, W. Debnam, C. Dalgard

    págs. 868-872

    

  • Generation of amplifiable genome-specific oligonucleotide probes and libraries

    I. Brukner, G.A. Tremblay, B. Paquin

    págs. 874-882

    

  • Comparison of probe preparation methods for DNA microarrays

    L. Eichinger, P. Farbrother, S. Müller, A.A. Noegel

    págs. 884-888

    

  • Increased yield of total RNA from fine-needle aspirates for use in expression microarray analysis

    W. Zhang, V. Dunmire, C. Wu, W.F. Symmans

    págs. 890-896

    

  • Production of bulk amounts of universal RNA for DNA microarrays

    T. Micsik, P. Van Hummelen, L.G. Puskás, A. Zvara, L. Hackler jr.

    págs. 898-904

    

  • Advantages of mRNA amplification for microarrays analysis

    M. Qian, F.M. Marincola, H.R. Alexander, S.K. Libutti, A.L. Feldman, N.G. Costouros, E. Wang

    págs. 906-914

    

  • Assessing sources of variability in microarray gene expression data

    B. Weir, S.E. Spruill, J. Lu, S. Hardy

    págs. 916-923

    

  • Use of RNA and genomic DNA references for inferred comparisons in DNA microarray analyses

    B.J. Haas, C.D. Town, J. Quackenbush, H. Kim, B. Zhao, E.C. Snesrud

    págs. 924-930

    

  • Fully automated radioligand binding filtration assay for membrane-bound receptors

    M. Melch, M. McConkey, J. Bergeron, S.K.-F. Wong, A. Shrikhande, C. Courtney, D. Smith

    págs. 932-937