BioTechniques

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  Año 2002, Vol. 33, Número 5

  • BioSpotlight

    pág. 961

    

  • Interruption of perfect CAG repeats by CAA triplets improves the stability of glutamine-encoding repeat sequences

    G.-J.B. Van Ommen, J.T. Den Dunnen, J.C. Dorsman, M. Bremmer-Bout, B. Pepers

    págs. 976-978

    

  • Gap repair transformation in fission yeast to exchange plasmid-selectable markers

    D.A. Kelly, C.S. Hoffman

    págs. 978-982

    

  • Excess dNTPs minimize RNA hydrolysis during reverse transcription

    G.F. Gerard, S. Collins, M.D. Smith

    págs. 984-990

    

  • Cloning method for taxonomic interpretation of T-RFLP patterns

    A. Mengoni, E. Grassi, Marco Bazzicalupo

    págs. 990-992

    

  • Molecular cloning using the interrupted recognition sequence of BplI to select for recombinant molecules

    M.A. Mastrodicasa, C.J. Brandl

    págs. 992-996

    

  • Analysis of L-dopa in human serum

    N. Dizdar, K. Arstrand, B. Kagedal

    págs. 1000-1002

    

  • Cryptic promoter activity of pBLCAT3 induced by overexpression of AP1 factors

    D.R. Edwards, I.M. Clark, M.-C. Hall, D.A. Young, A.D. Rowan

    págs. 1004-1008

    

  • Automated agarose gel electrophoresis of dsDNA fragments on a commercial DNA sequencer

    A. Guttman, J. Khandurina, E. Legg, X. Wang

    págs. 1008-1014

    

  • Quantitative nuclear run-off transcription assay

    L. Li, E.L. Chaikof

    págs. 1016-1017

    

  • Spotted substrates for focal presentation of proteins to cells

    J.R. Sanes, T.T. Kummer, H. Nishimune, R.O.L. Wong

    págs. 1018-1024

    

  • Automated colorimetric screen for apyrase inhibitors

    S.J. Roux, A.M. Lloyd, J.B. Windsor, C. Thomas, L. Hurley

    págs. 1024-1030

    

  • Creating random mutagenesis libraries using megaprimer PCR of whole plasmid

    K. Miyazaki, M. Takenouchi

    págs. 1033-1038

    

  • Broad-host-range mobilizable suicide vectors for promoter trapping in gram-negative bacteria

    W.J. Broughton, Hani Antoun, M. Izallalen, R. C. Levesque, X. Perret

    págs. 1038-1043

    

  • Detecting protein-protein interactions using Renilla luciferase fusion proteins

    P.D. Burbelo, A.E. Kisailus, J.W. Peck

    págs. 1044-1050

    

  • Negative purification method for the selection of specific antibodies from polyclonal antisera

    C. Benet, P. Van Cutsem

    págs. 1050-1054

    

  • Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria

    C.J. Marx, Mary E. Lidstrom

    págs. 1062-1067

    

  • Simulated microgravity culture system for a 3-D carcinoma tissue model

    E. Baba, N. Sato, K. Mizumoto, K. Sueishi, M. Tanaka, M. Katano, K. Nakamura, H. Kuga, T. Morisaki

    págs. 1068-1076

    

  • Quantitative real-time RT-PCR using hybridization probes and imported standard curves for cytokine gene expression analysis

    B.S. Kühne, P. Oschmann

    págs. 1078-1089

    

  • Ligation-mediated PCR: robotic liquid handling for DNA damage and repair

    A.D. Riggs, S.D. Flanagan, S.-M. Dai, T.R O'Connor, G.P Holmquist

    págs. 1090-1097

    

  • Prospects for new vaccines against tuberculosis

    L. Brandt, I. Orme

    págs. 1098-1102

    

  • Molecular haplotyping by Pyrosequencing (TM)

    M. Uhlen, J. Odeberg, K. Holmberg, P. Eriksson

    págs. 1104-1108

    

  • Multiplex universal genotyping using a modified ARMS-PCR protocol

    J.E. Vamvakopoulos

    págs. 1110-1112

    

  • Comparison of methods for determination of interleukin 6 G(-174)C promoter polymorphism

    C. Mannhalter, D. Häring, G. Endler, K. Hsieh

    págs. 1114-1117

    

  • Hemi-stranded SSCP analysis os SNPs in short sequence-tagged sites

    K. Hayashi, Y. Kukita, S. Manago, Shingo Baba

    págs. 1118-1121

    

  • Concurrent isolation of ribosomal, messenger, and low molecular weight RNA

    C.M. Stellrecht, V. Gandhi

    págs. 1122-1124

    

  • Fluorescence-based functional assay for Wnt/ß-catenin signaling activity

    R. Haydon, H. Cheng, Q. Zhou, B. Breyer, T. Feng, T.-C. He, L. Zhou, N. An, W. Jiang

    págs. 1126-1135

    

  • In vitro production and screening of DNA polymerase [eta] mutants for catalytic diversity

    E. Glick, J.P. Anderson, L.A. Loeb

    págs. 1136-1144

    

  • Comparison of Cis and Trans Tat gene expression in HIV LTR-based amplifier vectors

    T. Zhang, T.C. Tsang, D.T. Harris

    págs. 1146-1151

    

  • A pH-sensitive fluor, CypHer (TM) 5, used to monitor agonist-induced G protein-coupled receptor internalization in live cells

    M.J. Francis, A. Marenghi, M.E. Cooper, M. Briggs, N.P. Michael, G. Milligan, S. Game, E.J. Adie, S. Kalinka, L. Smith

    págs. 1152-1157