BioTechniques

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  Año 2000, Vol. 29, Número 5

  • Visualization of Nascent Transcripts on Drosophila Polytene Chromosomes Using BrUTP Incorporation

    M. L. Stevens, J. T. Westwood, W. Y. Chang, N. A. Winegarden, J. P. Paraiso

    págs. 934-935

    

  • Microarray-Based Detection of Select Cardiovascular Disease Markers

    R. C. McGlennen, G. Barany, N. E. Witowski, C. Leiendecker-Foster, N. P. Gerry

    págs. 936-943

    

  • Nonisotopic, PCR-Based Method for 5' Insulin Gene VNTR Allele Class Assignment

    J. A. Vazquez, L. Castaño, B. Calvo, J. R. Bilbao, G. P. de Nanclares

    págs. 944-947

    

  • Integration-Proficient Pseudomonas aeruginoas Vectors for Isolation of Single-Copy Chromosomal lacZ and lux Gene Fusions

    A. Becher, H. P. Schweizer

    págs. 948-953

    

  • pGSTp: An IVET-Compatible Promoter Probe Vector Conferring Resistance to Trimethoprim

    G. Shalom, J. G. Shaw, M. S. Thomas

    págs. 954-957

    

  • 3' Overhangs Influence PCR-SSCP Patterns

    R. Forrest, H. Zhou, J. G. H. Hickford

    págs. 958-961

    

  • Sequencing of beta~2-Adrenoceptor Gene PCR Products Using Taq BigDye™ Terminator Chemistry Results in Inaccurate Base Calling

    J. A. Johnson, L. M. Humma, W. G. Farmerie, M. R. Wallace

    págs. 962-969

    

  • Generation of Deletion and Point Mutations with One Primer in a Single Cloning Step

    O. Makarova, E. Kamberov, Ben Margolis

    págs. 970-975

    

  • Site-Directed Mutagenesis of Large (13-kb) Plasmids in a Single-PCR Procedure

    J. Wang, M. F. Wilkinson

    págs. 976-977

    

  • Construction of a cDNA Encoding a Repetitive Amino Acid Sequence

    T. Imamura, M. Asada, A. Yoneda, Y. Oda

    págs. 978-981

    

  • CTAB-Mediated Purification of PCR Products

    G. Zhang, J. H. Weiner

    págs. 982-987

    

  • Designing PCR Primers on the Web

    M. Binas

    págs. 988-996

    

  • Making Movies on a Weekend

    S. Paddock, D. McDougal

    págs. 997-1005

    

  • PCR Amplification Using Electrolytic Resistance for Heating and Temperature Monitoring

    D. M. Heap, M. G. Herrmann, C. T. Wittwer

    págs. 1006-1011

    

  • Nuclear Run-On Assay Using Biotin Labeling, Magnetic Bead Capture and Analysis by Fluorescence-Based RT-PCR

    M. Scaranari, I. Ceccherini, A. Puliti, R. Ravazzolo, G. Patrone, F. Puppo, R. Cusano

    págs. 1012-1017

    

  • Controlled Hot Start and Improved Specificity in Carrying Out PCR Utilizing Touch-Up and Loop Incorporated Primers (TULIPS)

    M. Ailenberg, M. Silverman

    págs. 1018-1023

    

  • Establishment and Chimera Analysis of 129/SvEv- and C57BL/6-Derived Mouse Embryonic Stem Cell Lines

    Q. Fang, A. B. Auerbach, D. Huszar, A. L. Joyner, W. Auerbach, J. H. Dunmore, V. Fairchild-Huntress

    págs. 1024-1033

    

  • Footprinting with an Automated Capillary DNA Sequencer

    W. Yindeeyoungyeon, M. A. Schell

    págs. 1034-1041

    

  • Fundamentals of DNA Hybridization Arrays for Gene Expression Analysis

    W. M. Freeman, D. J. Robertson, K. E. Vrana

    págs. 1042-1055

    

  • Detection of the Level of Estrogen Receptor and Functional Variants in Human Breast Cancers by Novel Assays

    Debajit K. Biswas, A. P. Cruz, A. B. Pardee

    págs. 1056-1065

    

  • Tissue-Specific Microdissection Coupled with ProteinChip® Array Technologies: Applications in Cancer Research

    W. Fiedler, K. Junker, U. Claussen, G. Ernst, F. von Eggeling, H. Davies, L. Lomas

    págs. 1066-1071

    

  • Pyrophosphorolysis-Activated Polymerization (PAP): Application to Allele-Specific Amplification

    Q. Liu, S. S. Sommer

    págs. 1072-1083

    

  • High-Accuracy DNA Sequence Variation Screening by DHPLC

    J. I. Spiegelman, M. N. Mindrinos, P.J. Oefner

    págs. 1084-1093

    

  • New Method for the Selection of Multicopy Transformants of Pichia pastoris, Using 3-Amino-1,2,4 Triazol

    J. Menendez, B. Garcia, Y. Hidalgo

    págs. 1094-1099

    

  • Generation of Monospecific Peptide Antibodies to the DNA Binding Domain of p53

    J. Hochman, M. Bergel, G. Tuchscherer, K. Huppi, D. Henderson, D. Siwarski

    págs. 1100-1107

    

  • Sensitive Method to Identify and Characterize Proteinases In Situ after SDS-PAGE

    J. Williams, W. J. McGrath, W. F. Mangel

    págs. 1108-1113

    

  • Perfusion System for Studying Peptide Secretion from Endocrine Cells with High Time Resolution

    E. Haug, B. C. Ostberg, O. Sand, T. Bjoro

    págs. 1114-1125

    

  • Small Molecule-Based Chemical Affinity System for the Purification of Proteins

    W. M. Ankener, M. L. Stolowitz, K. A. Hughes, J. D Chesnut, M. Bergseid, A. R. Baytan, J. P. Wiley

    págs. 1126-1133