BioTechniques

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  Año 2001, Vol. 30, Número 1

  • BioSpotlight

    págs. 9-19

    

  • Improved Methylation Protection-Based DNA Footprinting to Reveal Structural Distortion of DNA upon Transcription Factor Binding

    K. J. Reid, C. C. Nelson

    págs. 20-21

    

  • Background Activity of Reverse Transcriptases

    A. V. Timofeeva, N. A. Skrypina

    págs. 22-27

    

  • Development and Use of a Plasmid Encoding Green Fluorescent Protein in Multiple Antibiotic-Resistant Salmonella

    T. S. Frana, S. A. Carlson

    págs. 28-31

    

  • Large-Scale Colony Screening and Insert Orientation Determination Using PCR

    T. N. Campbell, F. Y. M. Choy

    págs. 32-35

    

  • Cost-Efficient Quantification of Enzyme-Linked Immunospot

    M. Bharadwaj, P. G. Parsons, D. M. Moss

    págs. 36-37

    

  • Microplate Assay for the Measurement of Hydroxyproline in Acid-Hydrolyzed Tissue Samples

    S. Brown, M. Worsfold, C. Sharp

    págs. 38-41

    

  • Use of DAPI Staining for Quantitation of Cell Chemotaxis

    D. English, G. Boguslawski, J. R. Grogg, K. A. Harvey

    págs. 42-49

    

  • Molecular Modeling on the Web

    S. Ranganathan

    págs. 50-59

    

  • System for Cleavable Fc Fusion Proteins Using Tobacco Etch Virus (TEV) Protease

    M. Grumet, J. Haspel, C. Blanco, J. Jacob

    págs. 60-65

    

  • Promoter Methylation Analysis on Microdissected Paraffin-Embedded Tissues Using Bisulfite Treatment and PCR-SSCP

    C. Fontolliet, J. Benhattar, Y.-S. Bian, P. Yan, M.-C. Osterheld

    págs. 66-73

    

  • Preparation of a Low Molecular Weight Polyethylenimine for Efficient Cell Transfection

    T. Bieber, H.-P. Elsasser

    págs. 74-80

    

  • Acetylcholinesterase Assay for Rapid Expression Screening in Liquid and Solid Media

    R. D. Schmid, F. Villatte, T. T. Bachman, A. S. Hussein

    págs. 81-87

    

  • Preparation of DNA Suitable for PCR Amplification from Fresh or Fixed Single Dinoflagellate Cells

    J. P. Abad, I. Marin, A. Aguilera, B. Reguera

    págs. 88-93

    

  • cDNA Library Screening Using the SOS Recruitment System

    B. de Crombrugghe, W. Huang, S.-l. Wang, G. Lozano

    págs. 94-101

    

  • Hybridization Screening of Very Short PCR Products for Paleoepidemiological Studies of Chagas' Disease

    A. C. Aufderheide, G. Fornaciari, C. Jaramillo, G. A. Vallejo, R. Yockteng, B. Arriaza, F. Cardenas-Arroyo, M. Madden, W. L. Salo, J. Streitz

    págs. 102-109

    

  • Spreadsheet-Based Program for the Analysis of DNA Methylation

    E. Gabrielson, R. Anbazhagan, J. G. Herman, K. Enika

    págs. 110-115

    

  • Microsoft® Word™ Macro for Analysis of Cytosine Methylation by the Bisulfite Deamination Reaction

    R. Singal, S. R. Grimes

    págs. 116-121

    

  • Conversion of Capillary Electrophoresis Microchip Genotyping Data for Analysis with Genetic Profiler™ Software

    J. R. Scherer, R. A. Mathies, G. J. Wedemayer, I. Medintz, W. W. Wong

    págs. 122-129

    

  • MouseBank: A Database Application for Managing Transgenic Mouse Breeding Programs

    R. Hopley, A. Zimmer

    págs. 130-133

    

  • Labeling DNA with Stable Isotopes: Economical and Practical Considerations

    P. Lecchi, F. P. Abramson, G. E. Black, Y. C. Boller, K. A. Kennedy

    págs. 134-141

    

  • Detection of Small Sequence Differences Using Competitive PCR: Molecular Monitoring of Genetically Improved, Mercury-Reducing Bacteria

    Y. Li, J. Lauber, J. Buer, Irene Wagner-Döbler, Andreas D. M. Felske, B. V. Pauling, H. F. von Canstein

    págs. 142-149

    

  • Quantitative Detection of Chlamydia spp. by Fluorescent PCR in the Light Cycler®

    P. Feng, T. Schlapp, B. Kaltenboeck, J. Huang, F. J. DeGraves, D. Gao

    págs. 150-157

    

  • Use of SMART™-Generated cDNA for Gene Expression Studies in Multiple Human Tumors

    P. D. Siebert, B. Zhumabayeva, L. Diatchenko, A. Chenchik

    págs. 158-163

    

  • Genes to Drugs

    T. K. Sawyer

    págs. 164-169

    

  • Current Concepts in Cancer Vaccine Strategies

    B. Monzavi-Karbassi, T. Kieber-Emmons

    págs. 170-189

    

  • Generation and Characterization of Recombinant Vascular Targeting Agents from Hybridoma Cell Lines

    P. E. Thorpe, C. Gottstein, W. Wels, B. Ober

    págs. 190-201

    

  • Maximization of Signal Derived from cDNA Microarrays

    P. W. Lane, P. J. Bugelski, S. E. Wildsmith, G. E. Archer, A. J. Winkley

    págs. 202-209

    

  • genotools SNP MANAGER: A New Software for Automated High-Throughput MALDI-TOF Mass Spectrometry SNP Genotyping

    Y. Stalgies, M. Kostrzewa, W. Pusch, K.-O. Kraeuter, T. Froehlich

    págs. 210-211