BioTechniques

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  Año 2008, Vol. 45, Número 3

  • Fom the editor

    págs. 203-203

    

  • Biospotlight

    págs. 205-205

    

  • Citations

    págs. 207-209

    

  • Scientists. Revealing the beauty of molecules. Preston J. MacDougall.

    L. Lederman

    págs. 211-211

    

  • Webwatch

    págs. 213-213

    

  • Technews. Stem cell technology.

    págs. 215-218

    

  • Tech insight. Curbing rampant cross-contamination and misidentification of cell lines.

    R.M. Nardone

    págs. 221-227

    

  • Troubleshooting forum

    págs. 229-229

    

  • NIH grant watch

    págs. 231-231

    

  • Biofeedback. Correction: Solid phase gene extraction isolates mRNA at high spatial and temporal resolution. Biotechniques 45

    P. Scherp

    págs. 233-233

    

  • Construction of a reading frame-independent yeast two-hybrid vector system for site-specific recombinational cloning and protein interaction screening

    J.W. Bauer, K. Önder, R.H. Maier, C.J. Brandner, H. Hintner

    págs. 235-244

    

  • Real-time PCR to determine transgene copy number and to quantitate the biolocalization of adoptively transferred cells from EGFP-transgenic mice

    K.M. Verbanac, M.U. Joshi, H.K. Pittman, C.E. Haisch

    págs. 247-258

    

  • An inexpresive and simple method for thermally stable immobilization of DNA on an unmodified glass surface: UV linking of poly(T)10-poly(C)10-tagged DNA probes

    A. Wolff, H. Gudnason, M. Dufva, D. Duong Bang

    págs. 261-271

    

  • Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences

    M.J. Heller, B. T. Messmer, J.Y. Marciniak, A.C. Kummel, S.C. Esener

    págs. 275-280

    

  • Assessment of repeated microarray experiments using mixed tissue RNA reference samples

    C.A. Afshari, H.K. Hamadeh, A. Mongan, M.A. Higgins, S. Pine

    págs. 283-292

    

  • Design of a single plasmid-based modified yeast one-hybrid system for investigation of in vivo protein-protein and protein-DNA interactions

    G. Chen, L.M. DenBoer, J.A. Shin

    págs. 295-304

    

  • Identification of highly expressed, soluble proteins using an improved, high-throughputpooled ORF expression technology

    R.M. Stephens, D.A. Lucas, J.L. Hartley, T.D. Veenstra, T. Waybright, W.K. Gillette, D. Esposito

    págs. 307-315

    

  • A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluerescein dye

    J.G. Rees, M. Meyer, M. Essack, S. Kanyanda

    págs. 317-320

    

  • Quantification of small cell numbers with a microchannel device

    D.J. Beebe, N.M. Badders, H. Yu, C.M. Alexander

    págs. 321-325

    

  • Plasmid sonication improves sequencing efficiency and quality in the Beckman Coulter CEQ system

    S.D. Rader, B.W. Murray, M.D. Thompson, K.G. Aukema, D. M. O'Bryan

    págs. 327-329

    

  • Real-time imaging of mitochondria in transgenic zebrafish expressing mitochondrially targeted GFP

    S.-Y. Choi, M. Jung Kim, K. Ho Kang, C.-H. Kim

    págs. 331-334

    

  • Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning

    B. Koebmann, P. Ruhdal Jensen, S. Braatsch, S. Helmark, H. Kranz

    págs. 335-337

    

  • Application Forum. A novel ex-vivo application of RNAi for neuroscience

    L. Baskin, S. Urschel, B. Eiberger

    págs. 338-339

    

  • Application Forum. A novel solid phase tecnology for high-throughput gene synthesis

    G. Horn, T. Waldmann, S. Arnold, M. Fuhrmann, O. Schatz, T. O'Connell, D. O'Connell, A. Auckenthaler, H. Schwer, J. Van den Brulle, M. Fischer, T. Langmann

    págs. 340-343

    

  • Application Forum. The art of selective killing: plasmid toxin/antitoxin systems and their technological applications.

    D. Stieber, P. Gabant, C.Y. Szpirer

    págs. 344-346

    

  • Application Forum. Dynamic imaging of calcium and STIM1 in the same cell using wide-field and TIRF microscopy.

    H. Llewelyn Roderick, S. Walker, N. Cunniffe, M. Bootman

    págs. 347-348

    

  • New Products

    págs. 349-352

    

  • Index to advertisers

    págs. 352-352