BioTechniques

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  Año 2004, Vol. 37, Número 5

  • BioSpotlight: In this issue

    págs. 707-709

    

  • Citations: Technical highlights of the recent literature

    pág. 711

    

  • Scientists: Profile of Ivar Giaever

    pág. 713

    

  • WebWatch

    pág. 715

    

  • Technology News: Separations Technologies

    págs. 716-717

    

  • Cost-effective trapezoidal modified Boyden chamber with comparable accuracy to a commercial apparatus

    K.C Lin, J.-Y Cheng, R.-H Chou, W.S. Chang, C.-W Wu

    págs. 724-726

    

  • Preparation of chimeric genes without subcloning

    R. Jerala, J. Vasl, G. Panter, M. Bencina

    págs. 726-730

    

  • High-throughput extraction of arthropod vector and pathogen DNA using bead milling

    P. Keim, D. M. Wagner , C.J. Allender, W.R. Easterday, M.N. Van Ert

    págs. 730-734

    

  • Simplified method for gene targeting vector construction

    F. Otto, M. Gasperowicz, M. Kolanczyk, M. Stock

    págs. 734-738

    

  • Improved expression vector for Brucella species

    G.G. Schuring, N. Sriraganathan, M.N. Seleem, R. Vemulapalli, S.M. Boyle

    págs. 740-744

    

  • Method based on electrophoresis and gel extraction for obtaining genomic DNA-free cDNA without DNase treatment

    A. Hohtola, L. Jaakola, A.M. Pirttilä, J. Vuosku

    págs. 744-748

    

  • LINE-1 insertion dimorphisms identification by PCR

    W. Pornthanakasem, A. Mutirangura

    págs. 750-752

    

  • Protocol for amplification of GC-rich sequences from Pseudomonas aeruginosa

    A. Raychaudhuri, P.A. Tipton

    págs. 752-756

    

  • BioFeedback

    págs. 758-723

    

  • Reliable method for generating double-stranded DNA vectors containing site-specific base modifications

    D. Brégeon, P.W. Doetsch

    págs. 760-766

    

  • In silico gene selection strategy for custom microarray desing

    V.R. Dondeti, C.W. Sipe, M.S. Saha

    págs. 768-776

    

  • PicoGmeter, a custom-made fluorometer for the quantification of dsDNA by PicoGreen (R) fluorescence

    G.G. Schofield

    págs. 778-782

    

  • BioMall

    págs. 784-788

    

  • Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslining

    K.C. Waldron, I. Migneault, C. Dartiguenave, M.J. Bertrand

    págs. 790-802

    

  • Fluorescence anisotropy microplate assay for analysis of steroid receptor-DNA interactions

    S.K. Nordeen, D.J. Shapiro, S.-Y. Wang, B.S. Ahn, Rob Harris

    págs. 807-817

    

  • High-throughput screening of suppression subtractive hybridization cDNA libraries using DNA microarray analysis

    D.K. Berger, N. Van den Berg, B.G Crampton, I. Hein , P.R.J. Birch

    págs. 818-824

    

  • Comparison of two probe preparation methods using long oligonucleotide microarrays

    F. Al-Mulla, R. Al-Tamimi, M.S. Bitar

    págs. 827-833

    

  • S-peptide epitope tagging for protein purification, expression monitoring, and localization in mammalian cells

    B.T. Vroman, L.M. Karnitz, S. H. Kaufmann , J.S. Hackbarth, S.-H. Lee, X.W. Meng

    págs. 835-839

    

  • "One plate/three-reporter" assay format for the detection and validation of yeast two-hybrid interactions

    J.F. Simons, D.R.H. Evans, K.A. Swirsding, B.E. Taillon

    págs. 840-843

    

  • Quenching accumulation of toxic galactose-1-phosphate as a system to select disruption of protein-protein interactions in vivo

    M. Petrascheck, A. Barberis, T. Gunde, S. Tanner, A. Auf der Maur

    págs. 844-852

    

  • Linear mRNA amplification from as little as 5 ng total RNA for global gene expression analysis

    M. Herrler, D. Iglehart, S. Koritala, S. Lato, S. Pillarisetty, R Purohit, M. Wang, S. Wang, N. Kurn, A. Dafforn, P. Chen, G. Deng

    págs. 854-857

    

  • New Products for Bioresearch

    págs. 858-862

    

  • Index to Advertisers

    pág. 863