PPM1F regulates ovarian cancer progression by afecting the dephosphorylation of ITGB1
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Yahui Leng [1] ; Zhenzi Luan [1] ; Zihang Li [1] ; Yongqing Ma [1] ; Yang Zhou [1] ; Jiaqi Liu [1] ; Song Liu [1] ; Tian Tian [1] ; Wenxiao Feng [1] ; Yanni Liu [1] ; Qin Shi [1] ; Chengyang Huang [1] ; Xuan Zhao [2] ; Wenlong Wang [1] ; Ao Liu [1] ; Tianhang Wang [1] ; Qiulei Ren [1] ; Jiakun Liu [1] ; Qian Huang [1] ; Yaling Zhang [1] ; Bin Yin [1] ; Jialin Chen [1] ; Liangliang Yang [1] ; Shiyun Zhao [1] ; Ruoyi Bao [1] ; Xingyu Ji [1] ; Yuewen Xu [1] ; Liaoyuan Liu [1] ; Junsuo Zhou [1] ; Miao Chen [1] ; Wenhui Ma [1] ; Li Shen [1] ; Te Zhang [1] ; Hongyan Zhao [1]
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[1] Hubei University of Medicine
Hubei University of Medicine
China
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[2] The Second Clinical College, Xi’an Medical University, Xi’an, China
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- Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 27, Nº. 3, 2025, págs. 1013-1025
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
PPM1F has been shown to play diverse biological functions in the progression of multiple tumors. PPM1F controls the T788/T789 phosphorylation switch of ITGB1 and regulates integrin activity. However, the impacts of PPM1F and ITGB1 on ovarian cancer (OV) progression remain unclear. Whether there is such a regulatory relationship between PPM1F and ITGB1 in ovarian cancer has not been studied. Therefore, the purpose of this study is to elucidate the function and the mechanism of PPM1F in ovarian cancer. The expression level and the survival curve of PPM1F were analyzed by databases. Gain of function and loss of function were applied to explore the function of PPM1F in ovarian cancer. A tumor formation assay in nude mice showed that knockdown of PPM1F inhibited tumor formation. We tested the efect of PPM1F on ITGB1 dephosphorylation in ovarian cancer cells by co-immunoprecipitation and western blotting. Loss of function was applied to investigate the function of ITGB1 in ovarian cancer. ITGB1-mut overexpression promotes the progression of ovarian cancer. Rescue assays showed the promoting efect of ITGB1-wt on ovarian cancer is attenuated due to the dephosphorylation of ITGB1-wt by PPM1F. PPM1F and ITGB1 play an oncogene function in ovarian cancer. PPM1F regulates the phosphorylation of ITGB1, which afects the occurrence and development of ovarian cancer.
