Parasagittal compartmentalization of the metabotropic glutamate receptor mGluR1b in the cerebellar cortex.

• Autores: R. Hawkes, Francisco Doñate Oliver, T. Knöpfel, J. M. Mateos, A. Osorio, R. Benítez Bermejo, Pedro Rolando Grandes Moreno, Jon Jatsu Azkue Barrenetxea, N. Puente, R. Kuhn
• Localización: European Journal of anatomy, ISSN-e 1136-4890, Vol. 5, Nº. 1, 2001, págs. 15-22
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • The parasagittal distribution of the splice variant 1b of the metabotropic glutamate receptor 1 (mGluR1b) was studied in the cerebellar cortex by using a specific mGluR1b antiserum combined with immunocytochemical methods. In f rontal cerebellar sections, the antibodies revealed alternating bands of immunopositive and immunonegative Purkinje cells in lobules I to X of the vermis. In these regions, the distribution of mGluR1b was complementary to longitudinal bands of Purkinje cells expressing Zebrin II. The width of the mGluR1b-positive bands d e c reased pro g ressively in the ro s t ro - c a u d a l direction, contrary to the rostro-caudal increase in the width of the Zebrin-positive bands. In the hemispheres, mGluR1b-positive bands alternated with Zebrin II-immunopositive bands in the paravermal portions of Crus I and Crus II. MGluR1b and Zebrin II immunoreactivities often colocalized in other regions of the hemispheral lobules. The compartmentalization of mGluR1b suggests that adjacent Purkinje cells might respond d i ff e rently depending on the presence of mGluR1b at parallel fiber-Purkinje cell synapses.