Localization of excitatory amino acid and neuropeptide markers in neurons of the subicular complex projecting to the retrosplenial granular cortex of the rat.

• Autores: Alicia Gonzalo Ruiz, I. Bayona
• Localización: European Journal of anatomy, ISSN-e 1136-4890, Vol. 5, Nº. 3, 2001, págs. 119-132
• Idioma: inglés
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• Resumen

  • Retrograde labelling was combined with immunohistochemistry to localize neurons containing glutamate and different neuropeptides such as neurotensin, leu-enkephalin, and substance P-like immunoreactivity in the projection pathways from the presubiculum and subiculum to the retrosplenial granular cortex of the rat. Injections of horseradish peroxidase conjugated to subunit B of cholera toxin (CT-HRP) into the retrosplenial granular cortex labelled large numbers of neurons in the presubiculum. A significant number of retrogradely-labelled neurons was seen in the dorsal subiculum, whereas small numbers of CT-HRP-labelled neurons were also found in the ventral subiculum. In the presubiculum, 90-95% of the CT-HRP-labelled neurons (30-32 per section) were also immunoreactive for glutamate, and small numb ers of re t rograd ely-labelled n eu ro ns also d isp layed neurotensin-, leu-enkephalin- or substance Pimmunoreactivity. In the subiculum, approximately 90-95% of the CT-HRP-labelled neurons (19-20 per section) were also immunoreactive for glutamate, and a significant number of retr ogradely- labelled neurons (70-75%, 14-15 per section) also displayed neurotensin immunoreactivity. In add ition , small numbers of CT-HRP-labelled neurons in the subiculum were immunoreactive for leu-enkephalin or substance P. These results suggest that the complexity of the neurotransmitter(s)/neuromodulator(s) of the subicular projections to the retrosplenial granular cortex of the rat should be taken into account when considering the mechanisms of the retr osplenial cortical neurons thought to play a role in memory