Resumen de Effects of Increasing Insulin Secretion on Acute Postexercise Blood Glucose Disposal

Ralph J. F. Manders, Arie Kies, Brechje Kaastra, Asker E. Jeukendrup, Eric van Breda

• Background: Coingestion of protein and/or free amino acids with carbohydrate has been reported to accelerate postexercise muscle glycogen synthesis due to an increase in the insulin response.

  Purpose: To determine the extent to which the combined ingestion of carbohydrate and a casein protein hydrolysate with or without additional free leucine can increase insulin levels during postexercise recovery in endurance-trained athletes. To determine how this affects whole-body plasma glucose disposal during postexercise recovery.

  Methods: Fourteen male athletes (age: 24.3 +/- 0.8 yr; [latin capital V with dot above]O2max: 62.9 +/- 1.4 mL[middle dot]kg-1[middle dot]min-1) were subjected to three randomized crossover trials in which they performed 2 h of exercise (55% Wmax). Thereafter, subjects were studied for 3.5 h during which they ingested carbohydrate (CHO: 0.8 g[middle dot]kg-1[middle dot]h-1), carbohydrate and a protein hydrolysate (CHO-PRO: 0.8 and 0.4 g[middle dot]kg-1[middle dot]h-1, respectively), or carbohydrate, a protein hydrolysate, and free leucine (CHO-PRO-LEU: 0.8, 0.4, and 0.1 g[middle dot]kg-1[middle dot]h-1, respectively) in a double-blind fashion. Continuous infusions with [6,6-2H2] glucose were applied to quantify plasma glucose appearance (Ra) and disappearance rates (Rd).

  Results: Plasma insulin responses were 108 +/- 17 and 190 +/- 33% greater in the CHO-PRO and CHO-PRO-LEU trial, respectively, compared with the CHO-trial (P < 0.01). Plasma glucose responses were lower in the CHO-PRO and CHO-PRO-LEU trial compared with the CHO-trial (35 +/- 5 and 42 +/- 11% lower, respectively; P < 0.01). Plasma glucose Ra and Rd were greater in the CHO versus the CHO-PRO and CHO-PRO-LEU trials (P < 0.05). Glucose Rd represented 100 +/- 0.03% of Ra in all trials.

  Conclusions: The combined ingestion of a protein hydrolysate and/or free leucine with carbohydrate (0.8 g[middle dot]kg-1[middle dot]h-1) substantially augments insulin secretion, but does not affect plasma glucose disposal during the first 3.5 h of postexercise recovery in trained athletes