The science of genetics has been revolutionized in recent years by the techniques of molecular biology, which make it possible to elucidate complex physiological functions at the gene level: especially after discovery of the Polymerase Chain Reaction (PCR), which enabled studying and measuring genetic variability at the molecular level. The utilization of molecular markers of the microsatellite type is especially noteworthy in this regard, because of the particular intrinsic properties which make them the markers of choice for both basic genetic studies and animal improvement The most important advances achieved in genetics based on information provided by these markers have been analysis of population genetics, creation of the most sophisticated gene maps, and detection of important genes. Measurement of DNA polymorphism by means of these genetic markers in organisms with complex genomes, has made possible the mapping, manipulation and cloning of genes associated with characters of biological interest. This article reviews some of the practical uses derived from identification and analysis of DNA polymorphism, especially those related to studies based on microsatellite-type markers. (Text in Spanish).
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