Lactoferrin Effect on Lymphocyte Cytokines and Apoptosis Is Independent of Exercise

• Autores: Paul A. Spagnuolo, Laurie Hoffman Goetz
• Localización: Medicine & Science in Sports & exercise: Official Journal of the American College of Sports Medicine, ISSN 0195-9131, Vol. 40, Nº. 6, 2008, págs. 1013-1021
• Idioma: inglés
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• Resumen

  • Background: Strenuous exercise increases apoptosis of intestinal lymphocytes (IL). Bovine lactoferrin (bLf), a protein found in milk products, affects lymphocyte apoptosis and the expression of TH1 and TH2 cytokines.

    Objective: The purpose was to determine whether bLf affects apoptosis and TH1 (tumor necrosis factor [alpha]) and TH2 (interleukin-10) cytokine expression in IL of mice given strenuous exercise.

    Methods: Female C57BL/6 mice (n = 89), given three bouts of treadmill exercise, were killed either immediately, 24 h after the last bout, or before initiation of exercise; within exercise conditions, mice were fed control (0% bLf) or bLf-supplemented (2% bLf) diet for 12 d until sacrifice. IL were enumerated, and apoptosis and cytokine expression were determined by Western blot analysis; markers of stress (corticosterone and iso-prostanes) were measured in the plasma by radioimmunoassay and direct immunoassay.

    Results: Exercise increased IL loss (P < 0.05) and the expression of caspase 3 (P < 0.001), heat shock protein 70 (P < 0.01), and interleukin-10 (P < 0.05) in mouse IL; bLf did not alter these responses. However, bLf reduced tumor necrosis factor [alpha] expression in mouse IL (P < 0.05), possibly through decreased nuclear factor [kappa]B expression (P < 0.05) in the supplemented group.

    Conclusions: Dietary bLf does not affect IL apoptosis after exercise but may confer intestinal protection through changes in cytokine expression, independent of exercise.