Genetic tags for labelling live cells: gap junctions and beyond

• Matthias M. Falk ^[1]
  1. [1] Scripps Research Institute

     Scripps Research Institute

     Estados Unidos

     
• Localización: Trends in cell biology, ISSN 0962-8924, Vol. 12, Nº 9, 2002, págs. 399-404
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • The availability of green fluorescent protein (GFP) as a tracer for observing proteins in living cells has revolutionized cell biology and spurred an intensive search for GFP variants with novel characteristics, additional autofluorescent proteins and alternative techniques of protein labelling. Two recent studies – one on tagging with tetracysteine motifs and labelling with biarsenic fluorophores of different colours, and the other on GFP tagging and fluorescence recovery after photobleaching (FRAP) – show how membrane channels are added and removed from gap junctions by using different fluorescent tags to distinguish between newly synthesized and older protein populations.