Influencia de la temperatura sobre las curvas de calibración en IRMA de IGF I. Interpretación mediante dos modelos no excluyentes

• Autores: T. Peris Conejero, R. Olivares Pallerols, José Luis Moreno Frigols
• Localización: Anales de la Real Academia Nacional de Farmacia, ISSN-e 1697-4298, ISSN 0034-0618, Nº. 4 3, 2008, págs. 369-378
• Idioma: español
• Títulos paralelos:
  • Influence of the temperature on calibration curves of IGF I in IRMA. Interpretation by two models non excluding
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• Resumen

  • The influence of temperature on the calibration graphs used in the analytical determination of IGF I by IRMA has been studied. The results were analyzed using the equation of the four parameters and a modification of the isotherm BET. Such equations correspond, respectively, to the models of interaction ligand (antigen) - receiver (antibody) and adsorption antigen on the wall of the tube coated antibody. The adjustments to both equations are satisfactory with some advantages for the adsorption model that make it preferable in the interpretation of results. It also determine thermodynamic parameters for the immunocomplex dissociation.