DNA recognition by the "Salmonella enterica" serovar Typhimurium transcription factor SlyA

• Autores: Fouzia Haider, James K. Lithgow, Melanie R. Stapleton, Valia A. Norte, Ruth E. Roberts, Jeffrey Green
• Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 11, Nº. 4, 2008, págs. 245-250
• Idioma: inglés
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• Resumen

  • The "Salmonella" regulatory protein SlyA is implicated in virulence, survival in macrophages and resistance to oxidative stress and anti-microbial peptides. SlyA is a member of the MarR family of winged-helix transcription factors.

    Systematic mutational analysis of the SlyA operator sequence and of the predicted DNA-binding region of SlyA shows that no single base pair in the palindromic SlyA operator sequence is essential for DNA binding, and identifies amino acid residues required to allow SlyA to recognise DNA. Combining the structure-function studies described here and elsewhere with the structures of MarR family proteins suggests a possible model for regulation of SlyA binding to DNA.