When one plus one equals more than two - a novel stain for renal biopsies is a combination of two classical stains

• Autores: Sergey V. Brodsky, Alia Albawardi, Anjali A. Satoskar, Gyongyi Nadasdy, Tibor Nadasdy
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 25, Nº. 11, 2010, págs. 1379-1383
• Idioma: inglés
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• Resumen

  • Histologic evaluation of renal biopsies includes multiple ancillary stains, including Periodic acid-Schiff�s (PAS) and Masson�s trichrome (Trichrome). Herein we report an innovative double-stain, derived from two standard stains (PAS and Trichrome). This novel stain not only has advantages of both ancestor stains, but became more distinguishable and colorful, when basement membranes stain dark-violet, whereas the interstitial collagen remains blue. This allows the pathologist immediate estimation of the amount of collagen, tubular atrophy and the degree of interstitial fibrosis in one section. Using computer-based analysis, we confirmed that our innovative double-stain highlights interstitial collagen better than Trichrome stain alone. We strongly recommend renal pathologists to try this innovative stain in their practice.