Comparison of BIOMED-2 Versus Laboratory-Developed Polymerase Chain Reaction Assays for Detecting T-Cell Receptor- Gene Rearrangements

• Autores: Keyur P. Patel
• Localización: The Journal of molecular diagnostics, ISSN 1525-1578, Vol. 12, Nº 2, 2010, págs. 226-237
• Idioma: inglés
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• Resumen

  • Detecting clonal T-cell receptor (TCR)-? gene rearrangements (GRs) is an important adjunct test for diagnosing T-cell lymphoma. We compared a recently described assay (BIOMED-2 protocol), which targets multiple variable (V) gene segments in two polymerase chain reaction (PCR) reactions (multi-V), with a frequently referenced assay that targets a single V gene segment in four separate PCR reactions (mono-V). A total of 144 consecutive clinical DNA samples were prospectively tested for T-cell clonality by PCR using laboratory-developed mono-V and commercial multi-V primer sets for TCR-? GR. The combination of TCR-?, mono-V TCR-? and multi-V TCR-? detected more clonal cases (68/144, 47%) than any individual PCR assay. We detected clonal TCR-? GR in 47/68 (69%) cases. Using either mono-V or multi-V TCR-? primers, the sensitivities for detecting clonality were 52/68 (76%) or 51/68 (75%). Using both mono-V and multi-V TCR-? primers improved the sensitivity for detecting clonality, 60/68 (88%). Combining either mono-V or multi-V TCR-? primers with TCR-? primers also improved the sensitivity, 64/68 (94%). Significantly, TCR-? V11 GRs could only be detected using the mono-V-PCR primers. We conclude that using more than one T-cell PCR assay can enhance the overall sensitivity for detecting T-cell clonality.