Isolation and characterization of carbendazim degrading "Trichoderma harzianum" rifai mutants

• Autores: Itamar Soares de Melo, Célia Maria Mahanhotto de S. Silva, Jane L. Faull
• Localización: Pesticidas: Revista de ecotoxicologia e meio ambiente, ISSN 0103-7277, Vol. 20, 2010, págs. 1-6
• Idioma: inglés
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• Resumen

  • Mutants of Trichoderma harzianum Rifai, obtained after ultraviolet (UV) light exposure, showed high resistant to the fungicide benomyl. A mutant (2B6) was capable of degrading carbendazim, other fungicide of the benzimidazole fungicide. This mutant degraded 41.5% of the molecule within five days. This and others mutants (2B1 and 2B2) presented variation in size and frequency of uni-nucleated and/or bi-nucleated spores compared to the wild type. Four primers generated RAPDs patterns that allowed the mutant to be differentiated from the wild-type. It is concluded that using UV mutagenization, it is feasible to obtain strains of T. harzianum with improved pesticide degradation ability.