Obtaining of Reducing Sugars from Kikuyu Grass (Pennisetum Clandestinum)
- Autores: Andrés Felipe López Vásquez, Gabriel Alberto Ortegón Rey, Fredy Antonio Robles Rodríguez
- Localización: Avances: Investigacion en Ingeniería, ISSN-e 2619-6581, ISSN 1794-4953, Vol. 1, Nº. 13 (Julio - Diciembre), 2010, págs. 98-101
- Idioma: inglés
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Resumen
The production of reducing sugars by acid and enzymatic hydrolysis of Kikuyu grass was performed in order to study reducing sugar production. The study involved hydrolysis of treated grass by immersed in water at environmental temperatures.
Acid hydrolysis was carried out through reflux boiling with different concentrations of diluted sulphuric acid (2%, 4%, 6% v/v) with in a liquid/ solid ratio of 30/1, while enzymatic hydrolysis was carried out with stirring in a water/enzyme (celluclast 1,5 LFG) solution. Both procedures were developed to different reaction times (2, 4 and 6 hrs). Reducing sugars concentrations were determined by UV-Vis spectrophotometry using the 3,5 dinitrosalisilic acid (DNS) method. Also, Benedict qualitative test confirmed reducing sugars presence in the samples. The greatest reducing sugars concentration was 2.5 g/l when using grass with aqueous treatment, with a 4 % sulfuric acid solution and 6 hours of reaction time. In general, results showed that greatest reducing sugars concentration were to high acid concentrations and reaction times.
