Objective: Artificial turf has been suggested as a risk factor for community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA). This is an experimental study looking at survival of CA-MRSA on artificial turf.
Methods: MRSA strain USA-300-0114 was grown as either planktonic cells or biofilms in liquid cultures of beef heart infusion broth overnight at 37°C. Beakers containing ProGrass (Pittsburgh, PA) turf were inoculated at the dirt interface with either ~5 × 107 planktonic bacteria or with biofilms. The inoculum included varying nutrient conditions consisting of spent medium, saline, or 5% mucin. The beakers were incubated at 37°C in ambient air. The main outcome measure was the number of surviving colony-forming units determined by plating on mannitol salt agar.
Results: Survival was biphasic with a colony-forming unit drop from ~5 × 107 to ~5 × 105 after the first week followed by survival of between 104 and 103 bacteria until termination of the experiment (20-50 d). Survival was dependent on nutrients, and washed cells survived less than 1 d. Mucin could serve as a nutrient source and slightly increased surviving numbers to 104-105 bacteria. Biofilm formation did not influence survival.
Conclusions: CA-MRSA survivability on artificial turf surfaces is dependent on the availability of nutrients. These results suggest that CA-MRSA could survive on artificial turf in significant numbers for 1 wk, and lower numbers for at least 1 month, if supplied with appropriate nutrients. Outdoor environmental conditions may affect these findings
© 2001-2024 Fundación Dialnet · Todos los derechos reservados