Genotype Variability and Clinical Features of Human Metapneumovirus Isolated from Korean Children, 2007 to 2010

• Autores: Hye Ryoun Kim, Ah Ra Cho, Mi Kyung Lee, Sin Weon Yun, Tae Hyoung Kim
• Localización: The Journal of molecular diagnostics, ISSN 1525-1578, Vol. 14, Nº. 1, 2012, págs. 61-64
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • This study was undertaken to determine the genotype variability of human metapneumovirus (hMPV) and its circulation pattern over a 3.5-year period, and to evaluate its clinical characteristics in Korean children. We investigated 4599 pediatric patients who were referred for a routine respiratory virus test by RT-PCR. hMPV genotype analyses were performed using a nested PCR�restriction fragment length polymorphism assay. Clinical and laboratory data obtained from medical records were reviewed retrospectively. Of the 4599 samples tested, 325 (7.1%) were positive for hMPV, and the co-infection rate among these 325 was 16%. Nested PCR�restriction fragment length polymorphism analysis clearly identified four of the five hMPV genotypes (A2a, A2b, B1, and B2) in 97.8%. The predominant genotype of hMPV changed over the 3.5-year study period from genotype A2a to B2 and then back to A2a. The most common genotype was A2a (214/325, 65.8%). Evidence of recurrent infection was obtained in one child only. Lymphocytosis was more frequent in children with a co-infection, but sputum production was less frequent than in children with a single infection. In genotype A2a hMPV�infected children, sneezing and neutrophilia were more frequent than in genotype B1 or B2 hMPV�infected children. This study broadens knowledge regarding the prevalence, the seasonal incidence, the occurrences of co-infection and re-infection, and the genotype diversity of hMPV in Korea.