Resumen de Efecto del medio de fecundación in vitro sobre el patron de reaccion acrosomica en el espermatozoide bovino

Juan Carlos Gardón, Joaquin Gadea

• español

  En este trabajo se ha analizado el proceso de reacción acrosómica de muestras congeladas de semen bovino cultivados en tres medios de cultivo utilizados para la fecundación in vitro TALP, TCM-199 y BO. El estado del acrosoma y la viabilidad espermática fue evaluada mediante una doble tinción fluorescente con lectinas unidas a fluoresceina y ioduro de propidio. El medio utilizado y el tiempo de cultivo ejercen un efecto significativo sobre los parámetros estudiados. Así el porcentaje de espermatozoides con acrosomas intactos es superior en el medio TCM-199 (valor medio 30.93±1.18) que en TALP (28.79±1.40) o BO (26.53±1.31). El medio TALP es el que induce un mayor porcentaje de espermatozoides con reacción acrosómica (valor medio TALP 9.33±0.81, TCM-199 6.76±0.64 BO 7.33±0.54), mientras que los espermatozoides cultivados en el medio TCM-199 presentan un retraso en el patrón de reacción acrosómica

• English

  Frozen bull semen was thawed and diluted in three different in vitro fertilization media: TCM-199, TALP and BO. Assessment of cytoplasmicc membrane integrity and proportion of acrosome reacted sperm were accomplished by FITC-PNA lectin and PI staining in a epi-fluorescent microscope. The results showed that the number of intact, acrosome reacted and damaged membrane spermatozoa was significantly affected by both IVF medium and time of culture (p<0.001). So, the number of sperm with intact acrosome and membrane was higher when was cultured in TCM-199 (mean value 30.93±1.18) than in TALP (28.79±1.40) and BO (26.53±1.31) and it was decreasing during the culture time. For the number of reacted acrosome spermatozoa was significantly higher for TALP than the others media and it was increasing during the culture period (mean value TALP 9.33±0.81, TCM-199 6.76±0.64, BO 7.33±0.54),. In relation to died sperm, BO was significantly higher than the others media and the relation between the number of acrosome reacted and total intact membrane spermatozoa was lower for TCM-199 than the other; finally, the process of acrosome reaction was slower than BO and TALP.