Resumen de Conservação do Sêmen da Carpa, Cyprinus carpio, Variedade Ornamenal, por Resfriamento.

Nilsa Hoffmann Padua, Eduardo Shimoda, Paula de Sousa Barbosa, Geraldo Pereira Junior

• English

  The objective of this study was to assess the feasibility of semen preservation of common carp, ornamental variety, in the refrigerator at 4 °C. Eight fish were used, pouring semen by abdominal massage. After application of crude extract of carp pituitary, the semen samples were extruded, and discarded the contaminated blood, feces and / or urine and showed that less than 80% motility. Each sample was divided into two tubes, one being maintained at room temperature (25 ± 3 °C) and the other kept in the refrigerator (4 ± 2 °C). For analysis of subjective motility, semen was pre-diluted by mixing one drop of semen + 4 drops of non-activating aqueous solution of NaCl 2%. The activation of the spermatozoa was performed by addition of 4 drops of distilled water to a drop of the mixture, since the microscope pre-focused. The analysis of sperm motility, both semen and kept at room temperature the stored under cooling, were taken every 1 hour. The motility of sperm (Y) is being reduced as a function of time held at room temperature (X) according to the equation: Y = 93.2 to 13.0. X (P<0.01; adjusted R ² = 81,7%). The cooled semen constant motility had a plateau of 91.5% motility for 4.4 hours, after which the spermatozoa (y) decreased with time under cooling (X), according to the equation: y = ± 130, 9 - 8.60. X (P<0.01, adjusted R ² = 79.7%). It was found that semen can be kept in the refrigerator until used for 11.7 hours.

• português

  O objetivo deste trabalho foi verificar a viabilidade da conservacao do semen da carpa comum, variedade ornamental, sob resfriamento a 4 oC. Foram utilizados oito peixes, vertendo semen mediante massagem abdominal. Apos a aplicacao de extrato bruto de hipofise de carpa, as amostras seminais foram extrusadas, sendo descartadas as contaminadas com sangue, fezes e/ou urina e as que apresentaram motilidade inferior a 80%. Cada amostra foi dividida em dois tubos de ensaio, sendo um deles mantido em temperatura ambiente (25 �} 3 oC) e o outro mantido sob resfriamento (4 �} 2 ��C). Para analise da motilidade direta subjetiva, o semen foi pre-diluido misturando-se 1 gota de semen + 4 gotas de solucao aquosa nao ativadora de NaCl a 2%. A ativacao dos espermatozoides foi efetuada mediante adicao de 4 gotas de agua destilada a uma gota da mistura, ja ao microscopio pre-focalizado. As analises de motilidade, tanto do semen mantido a temperatura ambiente quanto do conservado sob resfriamento, foram realizadas a cada 1 hora. A motilidade do semen (.) foi reduzindo-se, em funcao do tempo mantido a temperatura ambiente (X), segundo a equacao: .= 93,2 . 13,0.X (P<0,01; R2 ajustado = 81,7%). O semen resfriado apresentou motilidade constante num plato de 91,5% de motilidade por 4,4 horas, apos o qual a motilidade (.) caiu conforme transcorria o tempo sob resfriamento (X), de acordo com a equacao: . = + 130,9 - 8,60.X (P<0,01; R2 ajustado = 79,7%). Concluiu-se que o semen mantido sob resfriamento pode ser utilizado por ate 11,7 horas.