A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

• Autores: Reza Razeghifard, Catherine E. Chiafair, Dimitrios G. Giarikos
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 91, Nº 1, 2014, págs. 141-144
• Idioma: inglés
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• Resumen

  • A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene (BHT) by liver microsomes using gas chromatography-mass spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis, data interpretation of complex mixtures, and collaborative work.