Relationship between exhaled leukotriene and 8-isoprostane levels and asthma severity, asthma control level, and asthma control test score

• Autores: O. Keskin, S. Balaban, M. Keskin, E. Kucukosmanoglu, M Y Ozkars
• Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 42, Nº. 3, 2014, págs. 191-197
• Idioma: inglés
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• Resumen

  • Objective Exhaled breath condensate (EBC) is a completely non-invasive method for the collection of airway secretions to measure intense inflammation in the airways of asthmatics. It has been shown that the childhood asthma control test (c-ACT) is a good tool for use in the evaluation of asthmatics. Whether the c-ACT score and asthma control level correlate with the airway inflammation is not well known. We aimed to evaluate the relationship between exhaled cysteinyl leukotrienes (Cys-LTs) and 8-isoprostane levels and asthma severity, asthma control level and c-ACT score in asthmatic children.

    Methods Thirty asthmatic children were evaluated with c-ACT score and pulmonary function tests. Asthma severity and asthma control level were assessed according to GINA. EBC was collected and Cys-LTs and 8-isoprostane concentrations were determined using a specific immunoassay kit.

    Results Exhaled 8-isoprostane level in patients with moderate persistent asthma [114 (55�146) pg/ml] was higher than in the mild persistent group [52 (21�91) pg/ml] (p = 0.05, Mann�Whitney U [MWU]). EBC 8-isoprostane in children with 1�4 asthma exacerbations/year [52 (16�80) pg/ml] was significantly lower than in children with >4 asthma exacerbations/year [114 (57�129) pg/ml] (p < 0.05, MWU). No significant relation was determined between exhaled 8-isoprostane and Cys-LTs levels and c-ACT score and asthma control level. Exhaled 8-isoprostane correlated negatively with bronchodilator response (p = 0.015, r = -0.45).

    Conclusions Exhaled 8-isoprostane, as an oxidative stress specifier, was found to be increased in relation with asthma exacerbation frequency and oxidative stress increases with the severity of asthma. In contrast to asthma severity level, c-ACT score and asthma control level may not reflect airway inflammation.