Epigallocatechin-3-Gallate Attenuates Porphyromonas gingivalis Lipopolysaccharide-Enhanced Matrix Metalloproteinase-1 Production Through Inhibition of Interleukin-6 in Gingival Fibroblasts

• Autores: Wan-Chun Wen
• Localización: Journal of periodontology, ISSN 0022-3492, Nº. 9, 2014, págs. 868-875
• Idioma: inglés
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• Resumen

  • Background: Recent studies have shown that epigallocatechin-3-gallate (EGCG), a major constituent of green tea extract, exhibits effects of anti-inflammation and antioxidation on periodontal inflammation. The present in vitro study examines the effect of EGCG on Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS)�enhanced expression of interleukin (IL)-6 and matrix metalloproteinase (MMP)-1, as well as the activation of nuclear factor-kappa B (NF-?B). Furthermore, the role of IL-6 on LPS-enhanced MMP-1 production is evaluated using human gingival fibroblasts (HGFs).

    Methods: HGFs were primary cultured from human gingiva specimens. The cytotoxicities of EGCG and LPS were tested by cell viability tests. The cellular mRNA expression of IL-6 was determined by reverse-transcription polymerase chain reaction, and the protein expression of MMP-1 and IL-6 was examined by enzyme-linked immunosorbent assay. The cytosol expression and nuclear translocation of NF-?B was evaluated by immunocytochemistry followed by confocal laser scanning microscopy.

    Results: Pg LPS significantly increased MMP-1 production in HGFs, whereas adding EGCG significantly attenuated this enhanced production of MMP-1. LPS treatment also increased the mRNA and protein expression of IL-6 and stimulated NF-?B activation in HGFs. However, the addition of EGCG significantly attenuated the IL-6 expression and NF-?B activation. Supplemental addition of IL-6 significantly enhanced cellular MMP-1 production, whereas anti-IL-6 antibody inhibited LPS-enhanced MMP-1 production.

    Conclusion: EGCG could attenuate Pg LPS-enhanced production of MMP-1 in HGFs, whereas this attenuation might be due to the inhibition of IL-6 by EGCG.