Using Ion Exchange Chromatography To Separate and Quantify Complex Ions

• Autores: Brian J. Johnson
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 91, Nº 8, 2014, págs. 1212-1215
• Idioma: inglés
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• Resumen

  • Ion exchange chromatography is an important technique in the separation of charged species, particularly in biological, inorganic, and environmental samples. In this experiment, students are supplied with a mixture of two substitution-inert complex ions. They separate the complexes by ion exchange chromatography using a �flash� technique. After taking a visible spectrum of the eluted complexes, the unknowns are identified by comparison with a table of supplied ?max values and quantified using Beer�s law. This experiment can be completed in under 4 h and is suitable for introductory and intermediate chemistry students.