The separation of a mixture of myoglobin and blue dextran is explored using five different biochemical separation techniques (syringe filtration, Centricon, dialysis, gel filtration, and solid-phase extraction). The mixture is green and separation is detected by the visual observation of the colored components (orange�red myoglobin and blue dextran). Students gain exposure and hands-on practice with a broad range of modern biochemical separation techniques and the entire exercise can easily be completed in one laboratory period.
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