Illustrating the Concepts of Isotopes and Mass Spectrometry in Introductory Courses: A MALDI-TOF Mass Spectrometry Laboratory Experiment.

• Autores: Nancy Carter Dopke, Timothy Neal Lovett
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 84, Nº 12, 2007, págs. 1968-1970
• Idioma: inglés
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• Resumen

  • Mass spectrometry is a widely used and versatile tool for scientists in many different fields. Soft ionization techniques such as matrix-assisted laser desorption/ionization (MALDI) allow for the analysis of biomolecules, polymers, and clusters. This article describes a MALDI mass spectrometry experiment designed for students in introductory courses that provides hands-on experience with research instrumentation and reinforces the course concepts of isotopes, molecular masses, and molecular formulas. The students prepare peptide samples for mass analysis and then collect and analyze mass spectral data. This experiment could easily be adapted to an instructor's available or preferred soft ionization technique.