Resumen de Forensic Analysis of Canine DNA Samples in the Undergraduate Biochemistry Laboratory
Tobin M. Carson, Sharonda Q. Bradley, Brenda L. Fekete, Julie T. Millard, Frederick J. LaRiviere
Recent advances in canine genomics have allowed the development of highly distinguishing methods of analysis for both nuclear and mitochondrial DNA. We describe a laboratory exercise suitable for an undergraduate biochemistry course in which the polymerase chain reaction is used to amplify hypervariable regions of DNA from dog hair and saliva present at a "crime scene". Products are analyzed via gel electrophoresis to construct DNA profiles that implicate or exonerate dog owners under suspicion.
