Determination of Myoglobin Stability by Circular Dichroism Spectroscopy:: Classic and Modern Data Analysis

• Autores: Andrew F. Mehl, Mary A. Crawford, Lei Zhang
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 86, Nº. 5 (May), 2009, págs. 600-602
• Idioma: inglés
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• Resumen

  • Few laboratory procedures describe the use of circular dichroism (CD) at the undergraduate level. To increase the number of laboratory exercises using CD, a thermal denaturation study of myoglobin using CD is described to assess protein stability. Values obtained from a more classic linear data analysis approach are consistent with data analyzed with a modern software program that "fits" the data set to a model using a non-linear approach. Both methods gave quantities consistent with reported literature values.