Matrix Metalloproteinase-2, -9, and -12 Gene Polymorphisms in Generalized Aggressive Periodontitis

• Autores: Timur Kose, Afig Berdeli, Ali Gürkan, Gulnur Emingil, Buket Han, Gül Atilla, Serhat Çınarcık
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 78, Nº. 12, 2007, págs. 2338-2347
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Matrix Metalloproteinase-2, -9, and -12 Gene Polymorphisms in Generalized Aggressive Periodontitis Ali Gürkan,* Gülnur Emingil,* Buket Han Saygan,* Gül Atilla,* Serhat Çınarcık,* Timur Köse,† and Afig Berdeli‡ *Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey.

    †Department of Biostatistics and Medical Informatics, School of Medicine, Ege University.

    ‡Molecular Medicine Laboratory, Department of Pediatrics, School of Medicine, Ege University.

    Correspondence: Dr. Ali Gürkan, Department of Periodontology, School of Dentistry, Ege University, Bornova-35100, Izmir, Turkey. Fax: 90-232-3880325; e-mail: ali.gurkan@ege.edu.tr.

    Background: Matrix metalloproteinases (MMPs) are involved in periodontal tissue remodeling and degradation. Polymorphisms in the promoter region of the MMP-2 and -9 genes and in the coding region of the MMP-12 gene could affect transcription and the function of these enzymes. The aim of the present study was to determine the association between the aforementioned MMP polymorphisms and generalized aggressive periodontitis (GAgP).

    Methods: Genomic DNA was obtained from the peripheral blood of 92 subjects with GAgP and 157 periodontally healthy subjects. MMP-2 −735C/T, MMP-9 −1562C/T, and MMP-12 357Asn/Ser polymorphisms were genotyped by polymerase chain reaction and restriction fragment length polymorphism. Probing depth, clinical attachment loss, supragingival plaque accumulation, and bleeding on probing were recorded. The data were analyzed by χ2, logistic regression, and Mann-Whitney U tests.

    Results: The genotype distributions, allele frequencies, and rare allele carriage of MMP-2 and MMP-12 genes were similar in GAgP and healthy subjects (P >0.05). T allele frequency and T allele carriage of the MMP-9 −1562 C/T polymorphism were significantly lower in the GAgP group than in the healthy group (P <0.05). In addition, logistic regression analysis revealed a protective effect for MMP-9 −1562 T allele carriers (odds ratio = 0.52; P = 0.04).

    Conclusions: MMP-2 −735C/T and MMP-12 357Asn/Ser polymorphisms are not related to GAgP. Conversely, the MMP-9 −1562 gene T allele might be associated with a decreased risk for GAgP in the Turkish population.

    KEYWORDS: MMP-2, MMP-9, MMP-12, periodontitis, polymorphism Cited by Gülnur Emingil, Buket Han, Ali Gürkan, Afig Berdeli, Taina Tervahartiala, Tuula Salo, Pirkko J. Pussinen, Timur Köse, Gül Atilla and Timo Sorsa. (2014) Matrix Metalloproteinase (MMP)-8 and Tissue Inhibitor of MMP-1 (TIMP-1) Gene Polymorphisms in Generalized Aggressive Periodontitis: Gingival Crevicular Fluid MMP-8 and TIMP-1 Levels and Outcome of Periodontal Therapy. Journal of Periodontology 85:8, 1070-1080.

    Online publication date: 1-Aug-2014.

    Abstract | Full Text | PDF (729 KB) | PDF Plus (571 KB) Gwan Gyu Song, Jae-Hoon Kim and Young Ho Lee. (2013) Toll-like receptor (TLR) and matrix metalloproteinase (MMP) polymorphisms and periodontitis susceptibility: a meta-analysis. Molecular Biology Reports 40, 5129-5141.

    Online publication date: 1-Aug-2013.

    CrossRef Yongchu Pan, Dandan Li, Qi Cai, Weibing Zhang, Junqing Ma, Meilin Wang and Lin Wang. (2013) MMP-9 -1562C>T contributes to periodontitis susceptibility. Journal of Clinical Periodontology 40:10.1111/jcpe.2013.40.issue-2, 125-130.

    Online publication date: 1-Feb-2013.

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    CrossRef Yiu-fai Cheung, Wen-jing Hong, Koon-wing Chan and Sophia J. Wong. (2011) Modulating effects of matrix metalloproteinase-3 and -9 polymorphisms on aortic stiffness and aortic root dilation in patients after tetralogy of Fallot repair. International Journal of Cardiology 151, 214-217.

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    CrossRef A GURKAN, G EMINGIL, B SAYGAN, G ATILLA, S CINARCIK, T KOSE and A BERDELI. (2008) Gene polymorphisms of matrix metalloproteinase-2, -9 and -12 in periodontal health and severe chronic periodontitis. Archives of Oral Biology 53, 337-345.

    Online publication date: 1-Apr-2008.