Resumen de Inhibitory Effects of Incadronate on the Progression of Rat Experimental Periodontitis by Porphyromonas gingivalis Infection

Toshio Teranaka, Toshio Umemoto, Dr. Nobuyuki Tani-Ishii, Genshi Minamida, Daisuke Satoh, Keiko Chieda, Hiromasa Omuro, Akira Sugaya, Nobushiro Hamada, Yusuke Takahashi, Shiro Kiyohara, Isamu Kashima

• Inhibitory Effects of Incadronate on the Progression of Rat Experimental Periodontitis by Porphyromonas gingivalis Infection Dr. Nobuyuki Tani-Ishii Department of Operative Dentistry and Endodontics, Kanagawa Dental College, Kanagawa, Japan.

  Genshi Minamida Department of Operative Dentistry and Endodontics, Kanagawa Dental College, Kanagawa, Japan.

  Daisuke Saitoh Department of Operative Dentistry and Endodontics, Kanagawa Dental College, Kanagawa, Japan.

  Keiko Chieda Department of Operative Dentistry and Endodontics, Kanagawa Dental College, Kanagawa, Japan.

  Hiromasa Omuro Department of Periodontology, Kanagawa Dental College, Kanagawa, Japan.

  Akira Sugaya Department of Periodontology, Kanagawa Dental College, Kanagawa, Japan.

  Nobushiro Hamada Department of Oral Microbiology, Kanagawa Dental College, Kanagawa, Japan.

  Yusuke Takahashi Department of Oral Microbiology, Kanagawa Dental College, Kanagawa, Japan.

  Shiro Kiyohara Department of Oral Radiology, Kanagawa Dental College, Kanagawa, Japan.

  Isamu Kashima Department of Oral Radiology, Kanagawa Dental College, Kanagawa, Japan.

  Toshio Teranaka Department of Operative Dentistry and Endodontics, Kanagawa Dental College, Kanagawa, Japan.

  Toshio Umemoto Department of Oral Radiology, Kanagawa Dental College, Kanagawa, Japan.

  Background: Incadronate (YM175, disodium cycloheptylaminomethylenediphosphonate monohydrate), a bisphosphonate, has been suggested to prevent the bone resorption associated with periodontitis by inhibiting osteoclast activity. The purpose of this study was to investigate the effect of incadronate in preventing periodontal destruction in rats with Porphyromonas gingivalis-induced periodontitis.

  Methods: Periodontitis was induced in 35 Wister rats by inoculating P. gingivalis into the oral cavity and feeding the rats a soft diet for 4 weeks. Incadronate or placebo was administered to the oral cavity of the rats 2 days per week for 2, 4, or 8 weeks.

  Results: P. gingivalis infection resulted in destruction of the periodontal ligament, reduced bone density, and caused inflammatory cell migration. Radiographic, morphometric, and histological results showed that incadronate had the ability to increase the bone mineral density (quantum level score; cortex 518.9 [placebo 612.8]; sponge 579.8 [placebo 672.0]) and to prevent periodontal ligament destruction (width 0.16 mm [placebo 0.20 mm]; area 0.36 mm2 [placebo 0.54 mm2]) after 8 weeks' administration. Furthermore, the polymorphonuclear leukocyte (PMN) infiltration in gingival tissue was significantly decreased.

  Conclusion: These results showed that incadronate inhibits bone resorption and PMN migration in P. gingivalis-induced periodontitis. J Periodontol 2003;74:603-609.

  KEYWORDS: Animal studies, bisphosphonates/therapeutic use, bone resorption/prevention and control, incadronate/therapeutic use, neutrophils, periodontal ligament/pathology, periodontitis/prevention and control, Porphyromonas gingivalis.