Mandibular Bone Remodeling Under a Choline-Deficient Diet: A Histomorphometric Study in Rats

• Autores: Dr. Alejandro A. Gorustovich, María A. Espósito, María B. Guglielmotti, Máximo J. Giglio
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 74, Nº. 6, 2003, págs. 831-837
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Background: A deficiency of lipotropic factors in the rat induces renal, hepatic, and/or hematic damage. The aim of the present study was to evaluate the effect of a choline-deficient diet and refeeding on mandibular bone remodeling.

    Methods: Fifty Wistar rats were divided into 5 groups: group 1 (G1): control diet for 15 days; group 2 (G2): choline-deficient diet for 15 days; group 3 (G3): control diet for 30 days; group 4 (G4): choline-deficient diet for 30 days; and group 5 (G5): choline-deficient diet for 15 days and control diet for 15 days. All animals were sacrificed by ether overdose. The mandibles were resected, radiographed, decalcified, processed, and embedded in paraffin. Bucco-lingually oriented sections were obtained at the level of the interradicular bone of the medial roots of the left first molar, and stained with hematoxylin and eosin (H & E). Bone tissue density and bone remodeling were determined histomorphometrically. Body weight, food intake, hematocrit, and hemoglobinemia were also recorded.

    Results: Microscopic observation revealed that osteogenesis was lower in rats fed a choline-deficient diet, at both 15 and 30 days, and that this decrease did not revert with a control diet. Histomorphometric evaluation showed 37% and 27% reduction in bone tissue density at 15 and 30 days, respectively, and a 30% decrease in bone formation at 30 days, compared to controls.

    Conclusion: In this experimental model, a choline-deficient diet led to altered bone remodeling as observed by a marked reduction in osteogenesis. J Periodontol 2003;74:831-837.