FR 32. In vitro heterologous fertilization of buffalo oocytes

• Pedro Bastidas ^[1] ; Adriana Fernandez ^[1] ; Juan Troconiz ^[1]
  1. [1] Universidad Central de Venezuela

     Universidad Central de Venezuela

     Venezuela

     
• Localización: Archivos Latinoamericanos de Producción Animal, ISSN 1022-1301, ISSN-e 1022-1301, Vol. 5, Nº. Extra 3, 1997 (Ejemplar dedicado a: Proceedings XV ALPA meeting 2)
• Idioma: inglés
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• Resumen

  • This study was designed to evaluate both a homologous and heterologous system utilizing frozen semen Bos taurus, Bos indicus y Bos bubalis. Bovine and buffaloes ovaries were obtained from a slaughterhouse and transported to the laboratory in saline solution. A total of 120 bovine and 30 buffalo oocytes were collected from follicles of different sizes. They were cultured in a 35 mm petri dish containing TCM-199 for 24 h. There was a significant (P.05) due to type of semen. In conclusion, this study indicates that it is feasible to incorporate the bovine oocyte system as a laboratory tool to evaluate the fertility potential of semen from Bos indicus, Bos taurus and Bos bubalis breeds.