An Enzyme Kinetics Experiment for the Undergraduate Organic Chemistry Laboratory

• Robert J. Olsen ^[1] ; Julie A. Olson ^[1] ; Greta A. Giles ^[2]
  1. [1] Wabash College

     Wabash College

     Township of Union, Estados Unidos

     
  2. [2] State University, Dahlonega, Georgia
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 87, Nº 9 (September), 2010, págs. 956-957
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • An experiment using 1H NMR spectroscopy to observe the kinetics of the acylase 1-catalyzed hydrolysis of N-acetyl-dl-methionine has been developed for the organic laboratory. The l-enantiomer of the reactant is hydrolyzed completely in less than 2 h, and 1H NMR spectroscopic data from a single sample can be worked up to generate Michaelis−Menten and Lineweaver−Burke kinetic plots. The experiment introduces organic chemistry students to the use of a 1H NMR spectrometer, serves as an introduction to enzyme kinetics, and illustrates the stereospecificity of enzyme catalysis