A Comprehensive Enzyme Kinetic Exercise for Biochemistry

• Janice S. Barton ^[1]
  1. [1] Washburn University

     Washburn University

     City of Topeka, Estados Unidos

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 88, Nº 9, 2011, págs. 1336-1339
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • This article describes a comprehensive treatment of experimental enzyme kinetics strongly coupled to electronic data acquisition and use of spreadsheets to organize data and perform linear and nonlinear least-squares analyses, all in a manner that promotes development of important reasoning skills. Kinetic parameters are obtained for the stable enzyme bovine alkaline phosphatase by fitting the dependence of velocity on substrate and inhibitor concentration using regression algorithms of Excel.