Research in Undergraduate Instruction:: A Biotech Lab Project for Recombinant DNA Protein Expression in Bacteria

• Mark Brockman ^[1] ; Alfred B. Ordman ^[1] ; A. Malcolm Campbell ^[2]
  1. [1] Beloit College

     Beloit College

     City of Beloit, Estados Unidos

     
  2. [2] Davidson College

     Davidson College

     Estados Unidos

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 73, Nº 6 (June), 1996, págs. 542-543
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • In the sophomore-level Molecular Biology and Biotechnology course at Beloit College, students learn basic methods in molecular biology in the context of pursuing a semester-long original research project. We are exploring how DNA sequence affects expression levels of proteins. A DNA fragment encoding all or part of the guanylate monokinase (gmk) sequence is cloned into pSP73 and expressed in E. coli. A monoclonal antibody is made to gmk. The expression level of gmk is determined by SDS gel elctrophoresis, a Western blot, and an ELISA assay. Over four years, an increase in enrollment in the course from 9 to 34 students, the 85% of majors pursuing advanced degrees, and course evaluations all support the conclusion that involving students in research during undergraduate courses encourages them to pursue careers in science.