Simulation and Analysis of Differential Scanning Calorimetry Output:: Protein Unfolding Studies

• Babur Chowdhry ^[1] ; Stephen Leharne ^[1]
  1. [1] University of Greenwich

     University of Greenwich

     Reino Unido

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 74, Nº 2 (February), 1997, págs. 236-240
• Idioma: inglés
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• Resumen

  • High sensitivity differential scanning calorimetry ( HSDSC ) is a powerful and increasingly used technique for the study of molecular energetics in relation to biopolymers (e.g. proteins and nucleic acids ) or biomacromolecular assemblies ( e.g. liposomes ). HSDSC is, for example, the only method which allows thermodynamic parameters to be obtained directly for protein unfolding.The thermodynamic basis for HSDSC signals obtained for the equilibrium unfolding of proteins as well as the formalism for generating simulations of HSDSC outputs for protein unfolding are discussed. This will allow students ( studying biophysical chemistry, biochemistry, biophysics, molecular biology and allied subjects at undergraduate and postgraduate level ) to obtain an understanding of the type of data analysis required for HSDSC experiments on proteins.