“YFlag” – A Single-base Extension Primer Based Method for Gender Determination
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Julia S. Allwood [1] ; Sally Ann Harbison [2]
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[1] Mt Albert Science Centre Institute of Environmental Science and Research (ESR Ltd.) Auckland New Zealand The University of Auckland School of Chemical Sciences Auckland New Zealand
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[2] Mt Albert Science Centre Institute of Environmental Science and Research (ESR Ltd.) Auckland New Zealand
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- Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 60, Nº. 1, 2015, págs. 142-146
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Assigning the gender of a DNA contributor in forensic analysis is typically achieved using the amelogenin test. Occasionally, this test produces false-positive results due to deletions occurring on the Y chromosome. Here, a four-marker “YFlag” method is presented to infer gender using single-base extension primers to flag the presence (or absence) of Y-chromosome DNA within a sample to supplement forensic STR profiling. This method offers built-in redundancy, with a single marker being sufficient to detect the presence of male DNA. In a study using 30 male and 30 female individuals, detection of male DNA was achieved with c. 0.03 ng of male DNA. All four markers were present in male/female mixture samples despite the presence of excessive female DNA. In summary, the YFlag system offers a method that is reproducible, specific, and sensitive, making it suitable for forensic use to detect male DNA.
