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miR-448 negatively regulates ovarian cancer cell growth and metastasis by targeting CXCL12

  • Autores: Y. Lv, Y Lei, W Ding, C. Zhang, C Fang
  • Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 17, Nº. 11, 2015, págs. 903-909
  • Idioma: inglés
  • Texto completo no disponible (Saber más ...)
  • Resumen
    • Objectives The purpose of the study is to investigate the roles of miR-448 in ovarian cancer.

      Methods miR-448 and CXCL12 mRNA expression were examined using qRT-PCR. CXCL12 promoter activity was detected by luciferase activity system. Cell proliferation was assayed by MTT or colony formation. Migration and invasion was assayed by transwell chamber.

      Results miR-448 expression was usually under-expressed in ovarian cancer tissues and cell lines compared with their normal ones. Ectopic expression of miR-448 inhibited cell proliferation, migration and invasion in ovarian cancer cells. Moreover, bioinformatic prediction suggested that CXCL12 was a target gene of miR-448. We also demonstrated that restored expression of CXCL12 dampened miR-448-mediated suppression of tumor progression, which suggests the important role of miR-448 in tumor progression.

      Conclusion Our data indicate that miR-448 functions as a tumor suppressor in ovarian cancer, which exerts its activity by suppressing the expression of CXCL12.


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