Resumen de Effect of the methacrylate-based endodontic sealer Epiphany on rat peritoneal macrophages viability

Jenifer Martín González, Lizett Castellanos Cosano, Javier López Frias, Benito Sánchez Domínguez, Cristina Calvo Monroy, Luis Oscar Alonso Ezpeleta, Juan J. Segura Egea

• Objective:

  To evaluate the effect of the endodontic sealer Epiphany on rat peritoneal macrophages viability.

  Materials and methods:

  Peritoneal macrophages were obtained from Wistar rats and resuspended in RPMI- 1640 medium. Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of Epiphany, AH 26 and AH Plus sealers on RPMI-1640 medium were tested for cytotoxicity to rat peritoneal macrophages using the trypan blue dye exclusion assay. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level.

  Results:

  Crude extract of Epiphany killed 51% of cells, but was less cytotoxic that crude extracts of AH Plus and AH 26, which killed 81% and 86% of cells, respectively. Ten-fold dilutions of Epiphany, AH Plus and AH 26 killed 44%, 56%, 62% of macrophages, respectively. A hundred dilution of Epiphany only killed 7% of macrophages, but the same dilution of AH Plus and AH 26 killed 10% and 31% of macrophages, respectively. Lower dilutions of sealer extracts caused minimal cell death as compared to the control groups (p>0.05).

  Conclusions:

  The methacrylate-based endodontic sealer Epiphany showed lower cytotoxicity on macrophages than resin-based sealers AH Plus and AH 26. Dilution of elutes of the three materials by tenfold markedly reduced their effects