The Binding Constant for Complexation of Bilirubin to Bovine Serum Albumin. An Experiment for the Biophysical Chemistry Laboratory

• Kathryn R. Williams ^[1] ; Bhavin Adhyaru ^[1] ; Russell E. Pierce ^[1] ; Stephen G. Schulman ^[1]
  1. [1] University of Florida

     University of Florida

     Estados Unidos

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 79, Nº 1, 2002, pág. 115
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Students use fluorescence spectroscopy to measure the binding constant for bilirubin-bovine serum albumin complexation by the Method of Continuous Variations. The experiment applies fundamental concepts of physical chemistry to a commercially available system of biological interest. The experiment is easily completed in a single laboratory period, and students obtain binding constants on the order of 10 7, in good agreement with literature values.