Resumen de The Cis-Trans Equilibrium of N-Acetyl-L-Proline. An Experiment for the Biophysical Chemistry Laboratory
Kathryn R. Williams, Bhavin Adhyaru, Igor German, Eric Alvarez
Students in the biophysical chemistry laboratory use 1H NMR to evaluate the equilibrium constant for the cis-trans isomerism of N-acetyl-L-proline. The four solvent systems (45:55 d6-benzene-CDCl3, d6-acetone, D2O with pH < 2, and D2O with pH > 7) demonstrate the effects of solvent polarity and aqueous pH.
