The Influence of Selected Fingerprint Enhancement Techniques on Forensic DNA Typing of Epithelial Cells Deposited on Porous Surfaces
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Li-Chin Tsai [1] ; Cheng-Chang Lee [1] ; Chun-Chieh Chen [1] ; James Chun-I Lee [2] ; Sheng-Meng Wang [1] ; Nu-En Huang [4] ; Adrian Linacre [3] ; Hsing-Mei Hsieh [1]
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[1] Central Police University
Central Police University
Taoyuan District, Taiwán
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[2] National Taiwan University
National Taiwan University
Taiwán
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[3] Flinders University
Flinders University
Australia
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[4] Forensic Science Center New Taipei City Police Department New Taipei City Taiwan
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- Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 61, Nº. Extra 1, 2016, págs. 221-225
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Fingerprints deposited at crime scene can be a source of DNA. Previous reports on the effects of fingerprint enhancement methods have focused mainly on fingermarks deposited in blood or saliva. Here, we evaluate the effects of fingerprint enhancement methods on fingerprints deposited on porous surfaces. We performed real-time quantification and STR typing, the results of which indicated that two methods (iodine fuming and 1,2-indanedione in ethyl acetate enhancement) had no effect on the quantity of DNA isolated and resultant STR alleles when compared to control samples. DNA quantities and allele numbers were lower for samples enhanced with silver nitrate and 1,2-indanedione in acetic acid when compared to control samples. Based on DNA quantity, quality, and observable stochastic effects, our data indicated that iodine fuming and 1,2-indanedione in ethyl acetate were the preferred options for the enhancement of fingerprints on porous surfaces.
