Time-dependent Expression of MMP-2 and TIMP-2 after Rats Skeletal Muscle Contusion and Their Application to Determine Wound Age

• Tian-shui Yu ^[3] ; Zhuang Li ^[1] ; Rui Zhao ^[4] ; Yan Zhang ^[4] ; Zhen-hua Zhang ^[4] ; Da-wei Guan ^[2]
  1. [1] First Hospital of China Medical University

     First Hospital of China Medical University

     China

     
  2. [2] China Medical University

     China Medical University

     Taiwán

     
  3. [3] Collaborative Innovation Center of Judicial Civilization Beijing China
  4. [4] China University

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• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 61, Nº. 2, 2016, págs. 527-533
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • The ability to determine vitality and estimate the survival period after a wound is critical in routine forensic practice. The mRNA levels of MMP-2 and TIMP-2 were examined using quantitative real-time RT-PCR to determine the age of a wound. Furthermore, the colocalization of them with Macrophage Marker, respectively, was detected by double immunofluorescence, and a standardized rat model of skeletal muscle contusion was established. In the antemortem contused groups, a large number of macrophages showed positive staining for MMP-2 and TIMP-2, and the expression of MMP-2 and TIMP-2 mRNA increased sharply at 3 days postinjury, with relative quantities of 5.75 and 2.98. No samples in the other groups showed relative quantities of >5.75 and 2.98; therefore, relative quantities exceeding 5.75 and 2.98 were strongly indicated 3 days after contusion. In addition, there was a significant decrease in the relative quantity in the postmortem contused groups, indicating that they were useful for diagnosing vitality.